产品名称
干扰素β蛋白,重组人类, Recombinant Human Interferon Beta 1a (Hu-IFNbeta 1a).
biological source
human
recombinant
expressed in CHO cells
assay
≥95% (one N-glycosylation site)
form
liquid
specific activity
3.99 x 108 U/mg
manufacturer/tradename
Chemicon®
concentration
1 x 106 units/mL
technique(s)
cell culture | mammalian: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Quality Level
Analysis Note
比活度:通过检测病毒诱导的细胞病变的抑制作用来确定。在该干扰素抗病毒试验中,大约1单位/ml的干扰素是产生50%细胞病变效应所必需的量。这些单位是根据美国国立卫生研究院提供的人干扰素β(Hu-IFNβ)国际参考标准确定的。使用水泡性口炎病毒 (VSV) 测量 VERO 细胞的活性单位。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
产品来源:从CHO细胞中表达的人成纤维细胞mRNA中获得的cDNA。
重组人干扰素β 1a (Hu-IFNβ 1a)。 分子量约为 23 kDa。
Other Notes
替代:IF008
Physical form
50 mM NaOAc,pH 5.5,含有 0.1% BSA中的冷冻液体。
Preparation Note
接收后,该产品应在-70°℃或更低温度下保存,以保留全部活性。
用冷水孵育解冻产品瓶,直到刚解冻。解冻的IFNβ应
以工作等分试样保存于-70°C中,可保存长达六个月。避免多次冻融循环。
再冻结应在干冰或干冰/酒精浴中进行。
产品的进一步稀释应在含有蛋白质的缓冲液中进行,例如 0.1% 牛血清白蛋白 (BSA)。
用冷水孵育解冻产品瓶,直到刚解冻。解冻的IFNβ应
以工作等分试样保存于-70°C中,可保存长达六个月。避免多次冻融循环。
再冻结应在干冰或干冰/酒精浴中进行。
产品的进一步稀释应在含有蛋白质的缓冲液中进行,例如 0.1% 牛血清白蛋白 (BSA)。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Colton R Martens et al.
PLoS pathogens, 18(2), e1010342-e1010342 (2022-02-23)
Viral infection of the heart is a common but underappreciated cause of heart failure. Viruses can cause direct cardiac damage by lysing infected cardiomyocytes. Inflammatory immune responses that limit viral replication can also indirectly cause damage during infection, making regulatory
Fanning Zeng et al.
Cells, 9(12) (2020-12-10)
Genetic knock-in using homology-directed repair is an inefficient process, requiring the selection of few modified cells and hindering its application to primary cells. Here, we describe Homology independent gene Tagging (HiTag), a method to tag a protein of interest by
Zhaowei Chu et al.
Nature communications, 13(1), 1859-1859 (2022-04-08)
The cohesin complex participates in the organization of 3D genome through generating and maintaining DNA loops. Stromal antigen 2 (STAG2), a core subunit of the cohesin complex, is frequently mutated in various cancers. However, the impact of STAG2 inactivation on
Daniel Vogt et al.
Virology, 540, 150-159 (2020-01-14)
During DNA virus infections, detection of cytosolic DNA by the cGAS-STING pathway leads to activation of IFN-β. Kaposi's Sarcoma Herpesvirus (KSHV), an oncogenic DNA virus, is the etiological agent of Kaposi's Sarcoma, an endothelial cell (EC)-based tumor. To investigate the
Sarah Aref et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 28(4), 1043-1055 (2020-02-23)
The mechanism of tumor-selective replication of oncolytic measles virus (MV) is poorly understood. Using a stepwise model of cellular transformation, in which oncogenic hits were additively expressed in human bone marrow-derived mesenchymal stromal cells, we show that MV-induced oncolysis increased
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持