跳转至内容
Merck
CN
所有图片(1)

文件

IM27L

Sigma-Aldrich

Anti-Cathepsin B (Ab-1) Mouse mAb (CA10)

lyophilized, clone CA10, Calbiochem®

登录查看公司和协议定价


About This Item

UNSPSC代码:
12352203
NACRES:
NA.43

生物来源

mouse

质量水平

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

CA10, monoclonal

形式

lyophilized

不包含

preservative

种属反应性

porcine, human, bovine

请勿与下列物质发生反应

rat

制造商/商品名称

Calbiochem®

储存条件

OK to freeze

同位素/亚型

IgG2a

运输

ambient

储存温度

2-8°C

基因信息

mouse ... Ctsb(13030)

一般描述

Anti-Cathepsin B (Ab-1), mouse monoclonal, clone CA10, recognizes the ~43 kDa latent and ~31 kDa active forms of cathepsin B in HepG2 cells. It is validated for WB, IF, and IP.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/O-NS1 plasmacytoma cells. Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B.
Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B in HepG2 cells.

免疫原

Epitope: within the heavy chain of mature cathepsin B
Human
purified human liver cathepsin B

应用

Immunoblotting (250 ng/ml)

Immunoprecipitation (1 µg/ml)

Immunofluorescence (see comments)

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from 20 mM ammonium bicabonate buffer, 100 µg BSA (100 µg size) or 20 µg BSA (20 µg size).

重悬

Resuspend the lyophilized antibody with 1 ml sterile PBS. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product. Avoid freeze/thaw cycles.

分析说明

Positive Control
Hep G2 cells

其他说明

Sloane, B.F. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1:137-152.
Barret, A.J. 1987. Trends Biochem. Sci.12, 193.
Chan, S.J., et al. 1986. Proc. Natl. Acad. Sci. USA83, 7721.
Barrett, A.J. and Kirschke, H. 1981. Meth. Enzymol.80, 535.
This antibody recognizes an epitope on the heavy chain of mature cathepsin B and therefore detects both mature and pro-cathepsin B. Also reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Zhiyu Chen et al.
Cancer letters, 348(1-2), 156-166 (2014-03-26)
The tumor necrosis factor related apoptosis-inducing ligand (TRAIL) causes cancer cell death, but many cancers, including pancreatic cancer, are resistant to TRAIL therapy. A combination of TRAIL and the diterpene triepoxide, triptolide, is effective in inducing pancreatic cancer cell death.
Sonia Boulakirba et al.
Scientific reports, 8(1), 256-256 (2018-01-13)
CSF-1 and IL-34 share the CSF-1 receptor and no differences have been reported in the signaling pathways triggered by both ligands in human monocytes. IL-34 promotes the differentiation and survival of monocytes, macrophages and osteoclasts, as CSF-1 does. However, IL-34
Jun Zhang et al.
Cell death & disease, 8(7), e2954-e2954 (2017-07-28)
Although zinc oxide nanoparticles (ZnONPs) are widely used, they have raised concerns of toxicity in humans. Previous studies have indicated that reactive oxygen species (ROS) and autophagy are involved in the cytotoxicity of ZnONPs, but the regulatory mechanisms between autophagy
ZhengZheng Li et al.
Hepatology (Baltimore, Md.), 47(5), 1495-1503 (2008-01-29)
Impaired mitochondrial function is largely thought to be a core abnormality responsible for disease progression in nonalcoholic fatty liver disease (NAFLD). However, the molecular mechanisms resulting in mitochondrial dysfunction in NAFLD remain poorly understood. This study examined the effects of
Maksim V Baranov et al.
iScience, 11, 160-177 (2019-01-07)
Antigen presentation to T cells in major histocompatibility complex class II (MHC class II) requires the conversion of early endo/phagosomes into lysosomes by a process called maturation. Maturation is driven by the phosphoinositide kinase PIKfyve. Blocking PIKfyve activity by small molecule

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门