产品名称
Anti-MMP-7 (Ab-1) Mouse mAb (141-7B2), liquid, clone 141-7B2, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
141-7B2, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (5-10 µg/mL,
Paraffin Sections )
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MMP7(4316)
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
Analysis Note
Positive Control
CaR-1 or TPA treated SW620 cells
CaR-1 or TPA treated SW620 cells
Application

Immunoblotting (5-10 g/ml, see application references)
Paraffin Sections (see application references)
Disclaimer
Toxicity: Standard Handling (A)
General description
Purified mouse monoclonal antibody. Recognizes the ~28 kDa latent form of MMP-7.
Recognizes the ~28 kDa latent form of MMP-7 in TPA-treated SW620 cells. Does not recognize the active form of MMP-7.
This Anti-MMP-7 (Ab-1) Mouse mAb (141-7B2) is validated for use in Immunoblotting, Paraffin Sections for the detection of MMP-7 (Ab-1).
Immunogen
Human
human rectal carcinoma MMP-7
Other Notes
Brunner, K.L, et al. 1995. Proc. Natl. Acad. Sci. USA92, 7362.
Imai, K., et al. 1995. J. Biol. Chem.270, 6691.
Lichtinghagen, R., et al. 1995. Eur. J. Clin. Chem. Clin. Biochem.33, 65.
Nakano, A., et al. 1995. J. Neurosurg.83, 298.
Takino, T., et al. 1995. J. Biol. Chem.270, 23013.
Woessner, J.F. 1991. FASEB J.5, 2145.
Yamamoto, H., et al. 1995. J. Clin. Lab. Anal.9, 297.
Gaire, M., et al. 1994. J. Biol. Chem.269, 2032.
McDonnell, S., et al. 1994. Biochem. Soc. Trans.22, 58.
Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol.2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J.7, 1434.
Woessner, J.F. 1991. FASEB J.5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
Woessner, J.F., Jr and Taplin, C.J. 1988. J. Biol. Chem.263, 16918.
Sellers, A. and Woessner, J.F., Jr. 1980. Biochem. J.189, 521.
Imai, K., et al. 1995. J. Biol. Chem.270, 6691.
Lichtinghagen, R., et al. 1995. Eur. J. Clin. Chem. Clin. Biochem.33, 65.
Nakano, A., et al. 1995. J. Neurosurg.83, 298.
Takino, T., et al. 1995. J. Biol. Chem.270, 23013.
Woessner, J.F. 1991. FASEB J.5, 2145.
Yamamoto, H., et al. 1995. J. Clin. Lab. Anal.9, 297.
Gaire, M., et al. 1994. J. Biol. Chem.269, 2032.
McDonnell, S., et al. 1994. Biochem. Soc. Trans.22, 58.
Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol.2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J.7, 1434.
Woessner, J.F. 1991. FASEB J.5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
Woessner, J.F., Jr and Taplin, C.J. 1988. J. Biol. Chem.263, 16918.
Sellers, A. and Woessner, J.F., Jr. 1980. Biochem. J.189, 521.
Does not recognize the ~19 kDa active form of MMP-7. To detect active MMP-7, use Cat. No. IM47L. Does not cross-react with human MMP-1, MMP-2, MMP-3, MMP-8, MMP-9 or MMP-13. For optimal detection, SW620 cells should be treated with TPA (100 ng/ml) to induce expression of MMP-7. Antibody should be titrated for optimal results in individual systems.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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