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Merck
CN

IM40

Anti-MMP-7 (Ab-1) Mouse mAb (141-7B2)

liquid, clone 141-7B2, Calbiochem®

别名:

Anti-Matrilysin, Anti-Matrix Metalloproteinase 7, Anti-PUMP-1

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关于此项目

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
141-7B2, monoclonal
Species reactivity:
human
Application:
Citations:
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产品名称

Anti-MMP-7 (Ab-1) Mouse mAb (141-7B2), liquid, clone 141-7B2, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

141-7B2, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (5-10 µg/mL,
Paraffin Sections )

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

Gene Information

human ... MMP7(4316)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.

Analysis Note

Positive Control
CaR-1 or TPA treated SW620 cells

Application


Immunoblotting (5-10 g/ml, see application references)
Paraffin Sections (see application references)

Disclaimer

Toxicity: Standard Handling (A)

General description

Purified mouse monoclonal antibody. Recognizes the ~28 kDa latent form of MMP-7.
Recognizes the ~28 kDa latent form of MMP-7 in TPA-treated SW620 cells. Does not recognize the active form of MMP-7.
This Anti-MMP-7 (Ab-1) Mouse mAb (141-7B2) is validated for use in Immunoblotting, Paraffin Sections for the detection of MMP-7 (Ab-1).

Immunogen

Human
human rectal carcinoma MMP-7

Other Notes

Brunner, K.L, et al. 1995. Proc. Natl. Acad. Sci. USA92, 7362.
Imai, K., et al. 1995. J. Biol. Chem.270, 6691.
Lichtinghagen, R., et al. 1995. Eur. J. Clin. Chem. Clin. Biochem.33, 65.
Nakano, A., et al. 1995. J. Neurosurg.83, 298.
Takino, T., et al. 1995. J. Biol. Chem.270, 23013.
Woessner, J.F. 1991. FASEB J.5, 2145.
Yamamoto, H., et al. 1995. J. Clin. Lab. Anal.9, 297.
Gaire, M., et al. 1994. J. Biol. Chem.269, 2032.
McDonnell, S., et al. 1994. Biochem. Soc. Trans.22, 58.
Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol.2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J.7, 1434.
Woessner, J.F. 1991. FASEB J.5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
Woessner, J.F., Jr and Taplin, C.J. 1988. J. Biol. Chem.263, 16918.
Sellers, A. and Woessner, J.F., Jr. 1980. Biochem. J.189, 521.
Does not recognize the ~19 kDa active form of MMP-7. To detect active MMP-7, use Cat. No. IM47L. Does not cross-react with human MMP-1, MMP-2, MMP-3, MMP-8, MMP-9 or MMP-13. For optimal detection, SW620 cells should be treated with TPA (100 ng/ml) to induce expression of MMP-7. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

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存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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