生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
113-5B7, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human, rabbit, rat, mouse
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (10 µg/mL)
Immunofluorescence
Immunoprecipitation
Paraffin Sections )
同位素/亚型
IgG3
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... MMP14(4323)
一般描述
Purified mouse monoclonal antibody. Recognizes the ~60 kDa MTI-MMP protein in overexpressing cells.
Recognizes the ~60 kDa MTI-MMP protein in HT-1080 cells and in ConA-treated MD-MB-231 cells.
This Anti-MT1-MMP (Ab-4) Mouse mAb (113-5B7) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of MT1-MMP (Ab-4).
免疫原
Human
a synthetic peptide (CDGNFDTVAMLRGEM) corresponding to amino acids 319-333 in the hemopexin-like domain of human MT1-MMP
应用
Immunoblotting (10 g/ml)
Immunofluorescence (see application references)
Immunoprecipitation (see application references)
Paraffin Sections (see application references)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
外形
In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
HT-1080 or ConA-treated MD-MB-231 cells
HT-1080 or ConA-treated MD-MB-231 cells
其他说明
Sakakibara, M., et al. 1999. Cancer85, 231-239.
Mattei, M.G., et al. 1997. Genomics:40, 168.
Okada, A., et al. 1997. J. Cell. Biol.147, 67.
Okumura, Y., et al. 1997. FEBS Lett.402, 181.
Ueno, H., et al. 1997. Cancer Res.57, 2055.
Strongin, A.Y., et al. 1995. J. Biol. Chem.270 5331.
Takino, T., et al. 1995. J. Biol. Chem.270, 23013.
Cottam, D. W. and Rees, R. C., 1993. Intl J. Oncol.2, 861.
Stetler-Stevenson, W. G., et al. 1993. FASEB J.7, 1434.
Fridman, R., et al. 1992. J. Biol. Chem.267, 15389.
Woessner, J. F., 1991. FASEB J.5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G., 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
Goldberg, G.I., et al. 1989. PNAS86, 8207.
Mattei, M.G., et al. 1997. Genomics:40, 168.
Okada, A., et al. 1997. J. Cell. Biol.147, 67.
Okumura, Y., et al. 1997. FEBS Lett.402, 181.
Ueno, H., et al. 1997. Cancer Res.57, 2055.
Strongin, A.Y., et al. 1995. J. Biol. Chem.270 5331.
Takino, T., et al. 1995. J. Biol. Chem.270, 23013.
Cottam, D. W. and Rees, R. C., 1993. Intl J. Oncol.2, 861.
Stetler-Stevenson, W. G., et al. 1993. FASEB J.7, 1434.
Fridman, R., et al. 1992. J. Biol. Chem.267, 15389.
Woessner, J. F., 1991. FASEB J.5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G., 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
Goldberg, G.I., et al. 1989. PNAS86, 8207.
This antibody cross-reacts slightly with human MMP-3. To induce MT1-MMP in MD-MB-231 cells, treat with 1-5 µg/ml ConA for 24-48 h. Antibody should be titrated for optimal results in individual systems.
法律信息
Manufactured by Daiichi Fine Chemicals C0., Ltd., Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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