产品名称
蛋白质G Plus /蛋白质A琼脂糖悬浮液, Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.
form
slurry (Liquid)
contains
≤0.1% sodium azide as preservative
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
technique(s)
protein purification: suitable
suitability
suitable for microbiology
shipped in
wet ice
storage temp.
2-8°C
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Application
免疫沉淀法（参见评论）
Disclaimer
毒性：标准处理（A）
General description
专为免疫沉淀应用而设计。该产品已使用 BSA 进行封闭，以减少非特异性结合，不能用于纯化。
专门配制用于免疫沉淀的蛋白 G PLUS/蛋白 A 琼脂糖混合物。
Other Notes
提供琼脂糖溶液以备使用。蛋白 G Plus/蛋白 A 琼脂糖免疫沉淀试剂被 BSA 封闭，不应用于免疫球蛋白纯化或共价交联。对于免疫沉淀反应，建议每 µg 一抗加入 15 µl 溶液。预清除将最大程度地减少非特异性沉淀导致的多余条带。要进行预清除，将与免疫沉淀抗体相同物种的 20 µl 琼脂糖结合物和 1 µg 正常 IgG 加入样品中。当使用免疫印迹进行检测时，某些二抗可以与 BSA 或样品中存在的高浓度的其他蛋白质发生非特异性反应。这可以通过降低二抗的浓度来消除。
Physical form
PBS 中 33％ 的浆液。
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Cten is an oncogene promoting EMT in many signaling pathways, namely through Snail. We investigated whether Cten function could be mediated through Src. Cten levels were modulated by forced expression in HCT116 and gene knockdown in SW620 CRC (colorectal cancer)
E Clark et al.
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Productive high-titer infection by human immunodeficiency virus type 1 (HIV-1) requires the activation of target cells. Infection of quiescent peripheral CD4 lymphocytes by HIV-1 results in incomplete, labile reverse transcripts and lack of viral progeny formation. An interplay between Tat
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Oncology reports, 46(1) (2021-06-04)
Type 2 diabetes increases the risk various types of cancer and is associated with a poor prognosis therein. There is also evidence that the disease is associated with cancer metastasis. Centrosome amplification can initiate tumorigenesis with metastasis in vivo and increase
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We manufacture IP and antibody purification agarose beads that purify immunoglobulins and IgG fractions. Our kits also provide a total antibody purification process
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