IPFL00005
Immobilon® -FL PVDF Membrane
1 roll, 27 cm x 1.875 m, 0.45 µm pore size, transfer membrane with low background fluorescence
别名:
Western blotting membrane, blotting membrane, transfer membrane
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关于此项目
产品名称
Immobilon®-FL PVDF膜, 1 roll, 27 cm x 1.875 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
Quality Segment
material
PVDF membrane, plain filter, white filter
feature
hydrophobic
manufacturer/tradename
Immobilon®
technique(s)
dot blot: suitable, western blot: suitable
filter L × W
27 cm × 1.875 m
pore size
0.45 μm pore size
capacity
155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)
compatibility
for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
detection method
chemiluminescent, colorimetric, fluorometric
shipped in
ambient
Application
Features and Benefits
- 所有荧光染料
- 考马斯亮蓝
- 氨基黑
- 丽春红染色液
- CPTS
- Sypro™红宝石染色剂
Legal Information
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存储类别
10-13 - German Storage Class 10 to 13
实验方案
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。
商品
Troubleshoot protein transfer issues in Western blotting by understanding causes and optimizing transfer conditions.
相关内容
"Timeline of western blotting history. Timeless recipes and solutions for blotting success. Prepare and grow cell, media and sera with cultureware. Filter media with Stericup. ProteoExtract® kits for fractionation. CytoBuster™, BugBuster™ and CelLytic™ protein extraction reagents. Benzonase® nuclease. cOmplete™ and SIGMAFAST™ protease inhibitorsMaintain and preserve protein functionality following cell lysis using individual protease inhibitors and protease inhibitor cocktails. Amicon® filters for concentration and buffer exchange. PureProteome® magnetic beads for enrichment and purification. Recombinant protein purification affinity gels. Unlike methods that require careful aspiration to avoid sample loss, PureProteome® magnetic beads are isolated using a magnetic rack, for total removal of buffers and complete recovery with no sample dilution. TruPAGE™ precast gels Buffers (including Trizma®Tris base), solvents, prestained MW markers. Direct Detect® IR spectrometer for protein sample qualification. TruPAGE® precast gels are tear-resistant to reduce handling failures that commonly occur during gel electrophoresis and downstream processing. Range of Immobilon® PVDF transfer membranes. Western-validated antibodies. SNAP i.d.® 2.0 protein detection system. Immunodetection reagents. Blot efficiently using our Western blotting reagents, including Bløk® noise-cancelling reagents, Luminata™ Western HRP substrates, Immunoreaction Enhancer Reagents, and ReBlot™ Plus antibody stripping kits, all carefully optimized for your success."
Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| IPFL00005 | 04054839329791 |