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Merck
CN

IPFL00010

Millipore

Immobilon® -FL PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane with low background fluorescence

别名:

Western blotting membrane, blotting membrane, transfer membrane

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关于此项目

UNSPSC代码:
41105339
eCl@ss:
32031602
NACRES:
NB.22

主要文件

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一般描述

Immobilon®-FL膜是一种聚偏氟乙烯(PVDF)微孔膜，标称孔径为0.45 μm。它被设计用来结合从各种凝胶基质转移的蛋白质。Immobilon®-FL转移膜是疏水的，采用均匀的孔隙结构，具有高生物分子结合能力。该膜为分子量大于20,000的蛋白质提供了最佳印迹。Immobilon®-FL膜在很宽的激发/发射波长范围内显示非常低的自荧光。这种特性使其成为包括荧光免疫检测在内的印迹应用的理想材料。Immobilon®-FL膜也可用于标准化学发光或显色检测。

应用

Immobilon-FL PVDF膜已用于蛋白质印迹分析。

特点和优势

Immobilon-FL膜：
  • 首款为荧光应用优化的转移膜；极低的背景提高了所有荧光检测协议的灵敏度
  • 在广泛的激发/发射波长范围内兼容所有常用的荧光探针
  • 最适合多路复用和化学荧光检测
  • 兼容标准阻断剂和缓冲液

法律信息

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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S A Misek et al.
Oncogene, 39(7), 1466-1483 (2019-10-30)
Over half of cutaneous melanoma tumors have BRAFV600E/K mutations. Acquired resistance to BRAF inhibitors (BRAFi) remains a major hurdle in attaining durable therapeutic responses. In this study we demonstrate that ~50-60% of melanoma cell lines with vemurafenib resistance acquired in
Guangchuan Wang et al.
Biological research, 52(1), 60-60 (2019-12-19)
Recent studies have confirmed that RASAL1 has an antitumor effect in many cancers, but its functional role and the molecular mechanism underlying in colon cancer has not been investigated. We collected human colon cancer tissues and adjacent non-tumor tissues, human
David J Ferland et al.
PloS one, 15(2), e0229251-e0229251 (2020-02-25)
Since chemerin's identification as an adipokine, it has been associated with a number of human diseases including diabetes and obesity. However, the basic scientific foundation for these clinical determinations is still lacking. Fibroblastic mouse 3T3 cells are unable to develop
Enrique Calvo et al.
Science advances, 6(26), eaba7509-eaba7509 (2020-07-09)
Mitochondrial respiratory complexes assemble into supercomplexes (SC). Q-respirasome (III2 + IV) requires the supercomplex assembly factor (SCAF1) protein. The role of this factor in the N-respirasome (I + III2 + IV) and the physiological role of SCs are controversial. Here
Daniel Del Valle Morales et al.
Nucleic acids research, 48(7), 3806-3815 (2020-01-31)
Cap homeostasis is the cyclical process of decapping and recapping that maintains the translation and stability of a subset of the transcriptome. Previous work showed levels of some recapping targets decline following transient expression of an inactive form of RNMT
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