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Merck
CN

IPFL07810

Immobilon®-FL PVDF Membrane

1 roll, 7 x 8.4cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

别名:

Western blotting membrane, blotting membrane, transfer membrane

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UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red

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产品名称

Immobilon®-FL PVDF转移膜, 0.45 μm pore size, 7 X 8.4 cm sheets

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

7 cm × 8.4 cm

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red

detection method

chemiluminescent, colorimetric, fluorometric

shipped in

ambient

Quality Level

400

相关类别

General description

Immobilon®-FL转移膜是一种聚偏氟乙烯(PVDF)微孔膜，可结合从各种凝胶基质转移的蛋白质。Immobilon®-FL膜标称孔径为0.45 μm，建议用于印迹分子量大于20千道尔顿（kDa）的蛋白质。这种膜在很宽的激发/发射波长范围内显示非常低的自荧光。这种特性使其成为包括荧光免疫检测在内的印迹应用的理想材料。
过滤器代码：IPFL

Application

Immobilon®-FL转移膜已用于蛋白质印迹分析。

Features and Benefits

Immobilon®-FL转移膜：
  • 专为最佳荧光免疫检测应用而设计
  • 极低的背景提高了所有荧光检测协议的灵敏度
  • 在广泛的激发/发射波长范围内兼容所有常用的荧光探针
  • 可用于标准化学发光或显色检测

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Kevin K Caldwell et al.
Neurotoxicology and teratology, 66, 102-112 (2017-11-15)
Our previous studies suggest that prenatal arsenic exposure (50ppb) modifies epigenetic control of the programming of the glucocorticoid receptor (GR) signaling system in the developing mouse brain. These deficits may lead to long-lasting consequences, including deficits in learning and memory
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Zika virus (ZIKV) infection is endemic to several world regions, and many others are at high risk for seasonal outbreaks. Synthetic DNA-encoded monoclonal antibody (DMAb) is an approach that enables in vivo delivery of highly potent mAbs to control infections. We
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Valproic acid (VPA) is the most widely prescribed antiepileptic drug due to its ability to treat a broad spectrum of seizure types. However, potential complications of this drug include anticonvulsant polytherapy metabolism, organ toxicity and teratogenicity which limit its use

商品

荧光标记抗体_荧光WesternBlot-默克生命科学

荧光Western blot检测因具有多重检测的方便性而变得越来越受研究者的欢迎，但这种检测仍需要专门优化的工具来获得理想的实验结果。这里我们推荐一个最佳组合，以帮助大家在荧光检测中快速获得稳定、易重复的数据。

相关内容

Antibody recovery and reuse in the SNAP i.d.®2.0 immunodetection system

Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.

SNAP i.d. 2.0 System Brochure

There’s so much room for experimental variability in traditional immunodetection workflows. For your peace of mind – and ours – we designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry experiments. The concept is simple: a vacuum-driven flow of blocking, antibody, and washing solutions reduces slide and membrane handling. That means a lot less shaking, dipping, pouring and waiting. And now you can process multiple blots and slides in parallel, so it’s easy to apply consistent conditions across experiments.

User Guide - Immobilon® FL Transfer Membrane
Immobilon®-E Transfer Membrane User Guide
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