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Merck
CN

IPFL10100

Immobilon® -FL PVDF Membrane

10 sheets, 10 cm x 10 cm, 0.45 µm pore size, transfer membrane with low background fluorescence

别名:

Western blotting membrane, blotting membrane, transfer membrane

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关于此项目

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22

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产品名称

Immobilon®-FL PVDF膜, 10 sheets, 10 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

material

PVDF membrane
plain filter
white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

10 cm × 10 cm

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin)
 205 μg/cm2 adsorption capacity (BSA)
 300 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red

detection method

chemiluminescent
colorimetric
fluorometric

shipped in

ambient

Quality Level

400

相关类别

Application

Immobilon®-FL膜已被用于蛋白质印迹。它也适用于标准化学发光或显色检测。

Features and Benefits

Immobilon®-FL膜：
  • 为荧光应用设计的第一种转移膜极低的背景提高了所有荧光检测方案的灵敏度
  • 在所有激发和发射波长下与所有常用的荧光探针兼容
  • 与标准阻断剂和缓冲液兼容
  • 适用于多路传输和化学荧光检测

General description

Immobilon®-FL转移膜是疏水性聚偏氟乙烯（PVDF）微孔膜，用于从各种凝胶基质转移蛋白质。这种转移膜提供了一种均匀控制的孔结构，对生物分子具有高的结合能力。该膜在宽范围的激发/发射波长上显示出非常低的自发荧光。Immobilon®-FL膜的标称孔径为0.45 μm，并为分子量大于20000道尔顿的蛋白质提供最佳的印迹。

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Jessica E Chukwu et al.
Scientific reports, 8(1), 6209-6209 (2018-04-20)
Tau antibodies have shown therapeutic potential for Alzheimer's disease and several are in clinical trials. As a microtubule-associated protein, tau relies on dynamic phosphorylation for its normal functions. In tauopathies, it becomes hyperphosphorylated and aggregates into toxic assemblies, which collectively
Yerim Lee et al.
eLife, 8 (2019-11-02)
Membrane nanodomains have been implicated in Ras signaling, but what these domains are and how they interact with Ras remain obscure. Here, using single particle tracking with photoactivated localization microscopy (spt-PALM) and detailed trajectory analysis, we show that distinct membrane
Maria Consiglia Trotta et al.
PloS one, 14(1), e0211005-e0211005 (2019-01-19)
The role of the hydroxycarboxylic acid receptor 2 (HCA2) in the retinal damage induced by diabetes has never been explored. In this context, the present study highlights an upregulation of retinal HCA2 receptors in diabetic C57BL6J mice. Moreover, we illustrate
Shane M Bemiller et al.
Journal of neuroinflammation, 15(1), 278-278 (2018-09-27)
Fractalkine (CX3CL1) and its receptor (CX3CR1) play an important role in regulating microglial function. We have previously shown that Cx3cr1 deficiency exacerbated tau pathology and led to cognitive impairment. However, it is still unclear if the chemokine domain of the
Rosa Maisto et al.
Frontiers in pharmacology, 11, 235-235 (2020-03-27)
Stimulation of retinal photoreceptors with elevated glucose concentration (30 mM) for 96 h, served as diabetic retinopathy in vitro model to study Resolvin D1 (50 nM) effects on neovascularization. VEGF and anti-angiogenic miR-20a-3p, miR-20a-5p, miR-106a-5p, and miR-20b expression was assessed
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荧光标记抗体_荧光WesternBlot-默克生命科学

荧光Western blot检测因具有多重检测的方便性而变得越来越受研究者的欢迎，但这种检测仍需要专门优化的工具来获得理想的实验结果。这里我们推荐一个最佳组合，以帮助大家在荧光检测中快速获得稳定、易重复的数据。

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