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Merck
CN
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文件

IPFL10100

Millipore

Immobilon® -FL PVDF Membrane

10 sheets, 10 cm x 10 cm, 0.45 µm pore size, transfer membrane with low background fluorescence

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别名:
Western blotting membrane, blotting membrane, transfer membrane
UNSPSC代码:
41105339
eCl@ss:
32031602
NACRES:
NB.22

product name

Immobilon®-FL PVDF膜, 10 sheets, 10 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

物料

PVDF membrane
plain filter
white filter

质量水平

400

特点

hydrophobic

制造商/商品名称

Immobilon®

技术

dot blot: suitable
western blot: suitable

过滤器长度 × 宽度

10 cm × 10 cm

孔径

0.45 μm pore size

容量

155 μg/cm2 adsorption capacity (insulin)
 205 μg/cm2 adsorption capacity (BSA)
 300 μg/cm2 adsorption capacity (goat IgG)

相容性

for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red

检测方法

chemiluminescent
colorimetric
fluorometric

运输

ambient

相关类别

一般描述

Immobilon®-FL转移膜是疏水性聚偏氟乙烯（PVDF）微孔膜，用于从各种凝胶基质转移蛋白质。这种转移膜提供了一种均匀控制的孔结构，对生物分子具有高的结合能力。该膜在宽范围的激发/发射波长上显示出非常低的自发荧光。Immobilon®-FL膜的标称孔径为0.45 μm，并为分子量大于20000道尔顿的蛋白质提供最佳的印迹。

应用

Immobilon®-FL膜已被用于蛋白质印迹。它也适用于标准化学发光或显色检测。

特点和优势

Immobilon®-FL膜：
  • 为荧光应用设计的第一种转移膜极低的背景提高了所有荧光检测方案的灵敏度
  • 在所有激发和发射波长下与所有常用的荧光探针兼容
  • 与标准阻断剂和缓冲液兼容
  • 适用于多路传输和化学荧光检测

法律信息

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

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Jessica E Chukwu et al.
Scientific reports, 8(1), 6209-6209 (2018-04-20)
Tau antibodies have shown therapeutic potential for Alzheimer's disease and several are in clinical trials. As a microtubule-associated protein, tau relies on dynamic phosphorylation for its normal functions. In tauopathies, it becomes hyperphosphorylated and aggregates into toxic assemblies, which collectively
Yerim Lee et al.
eLife, 8 (2019-11-02)
Membrane nanodomains have been implicated in Ras signaling, but what these domains are and how they interact with Ras remain obscure. Here, using single particle tracking with photoactivated localization microscopy (spt-PALM) and detailed trajectory analysis, we show that distinct membrane
Maria Consiglia Trotta et al.
PloS one, 14(1), e0211005-e0211005 (2019-01-19)
The role of the hydroxycarboxylic acid receptor 2 (HCA2) in the retinal damage induced by diabetes has never been explored. In this context, the present study highlights an upregulation of retinal HCA2 receptors in diabetic C57BL6J mice. Moreover, we illustrate
Shane M Bemiller et al.
Journal of neuroinflammation, 15(1), 278-278 (2018-09-27)
Fractalkine (CX3CL1) and its receptor (CX3CR1) play an important role in regulating microglial function. We have previously shown that Cx3cr1 deficiency exacerbated tau pathology and led to cognitive impairment. However, it is still unclear if the chemokine domain of the
Rosa Maisto et al.
Frontiers in pharmacology, 11, 235-235 (2020-03-27)
Stimulation of retinal photoreceptors with elevated glucose concentration (30 mM) for 96 h, served as diabetic retinopathy in vitro model to study Resolvin D1 (50 nM) effects on neovascularization. VEGF and anti-angiogenic miR-20a-3p, miR-20a-5p, miR-106a-5p, and miR-20b expression was assessed
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荧光标记抗体_荧光WesternBlot-默克生命科学

荧光Western blot检测因具有多重检测的方便性而变得越来越受研究者的欢迎，但这种检测仍需要专门优化的工具来获得理想的实验结果。这里我们推荐一个最佳组合，以帮助大家在荧光检测中快速获得稳定、易重复的数据。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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