跳转至内容
Merck
CN

IPFL10100

Immobilon® -FL PVDF Membrane

10 sheets, 10 cm x 10 cm, 0.45 µm pore size, transfer membrane with low background fluorescence

别名:

Western blotting membrane, blotting membrane, transfer membrane

登录 查看组织和合同定价。

选择尺寸

关于此项目

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane
plain filter
white filter
Detection method:
chemiluminescent
colorimetric
fluorometric
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

产品名称

Immobilon®-FL PVDF膜, 10 sheets, 10 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

material

PVDF membrane
plain filter
white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

10 cm × 10 cm

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin)
 205 μg/cm2 adsorption capacity (BSA)
 300 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red

detection method

chemiluminescent
colorimetric
fluorometric

shipped in

ambient

Quality Level

400

相关类别

Application

Immobilon®-FL膜已被用于蛋白质印迹。它也适用于标准化学发光或显色检测。

Features and Benefits

Immobilon®-FL膜：
  • 为荧光应用设计的第一种转移膜极低的背景提高了所有荧光检测方案的灵敏度
  • 在所有激发和发射波长下与所有常用的荧光探针兼容
  • 与标准阻断剂和缓冲液兼容
  • 适用于多路传输和化学荧光检测

General description

Immobilon®-FL转移膜是疏水性聚偏氟乙烯（PVDF）微孔膜，用于从各种凝胶基质转移蛋白质。这种转移膜提供了一种均匀控制的孔结构，对生物分子具有高的结合能力。该膜在宽范围的激发/发射波长上显示出非常低的自发荧光。Immobilon®-FL膜的标称孔径为0.45 μm，并为分子量大于20000道尔顿的蛋白质提供最佳的印迹。

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Yerim Lee et al.
eLife, 8 (2019-11-02)
Membrane nanodomains have been implicated in Ras signaling, but what these domains are and how they interact with Ras remain obscure. Here, using single particle tracking with photoactivated localization microscopy (spt-PALM) and detailed trajectory analysis, we show that distinct membrane
Jessica E Chukwu et al.
Scientific reports, 8(1), 6209-6209 (2018-04-20)
Tau antibodies have shown therapeutic potential for Alzheimer's disease and several are in clinical trials. As a microtubule-associated protein, tau relies on dynamic phosphorylation for its normal functions. In tauopathies, it becomes hyperphosphorylated and aggregates into toxic assemblies, which collectively
Maria Consiglia Trotta et al.
PloS one, 14(1), e0211005-e0211005 (2019-01-19)
The role of the hydroxycarboxylic acid receptor 2 (HCA2) in the retinal damage induced by diabetes has never been explored. In this context, the present study highlights an upregulation of retinal HCA2 receptors in diabetic C57BL6J mice. Moreover, we illustrate
Shane M Bemiller et al.
Journal of neuroinflammation, 15(1), 278-278 (2018-09-27)
Fractalkine (CX3CL1) and its receptor (CX3CR1) play an important role in regulating microglial function. We have previously shown that Cx3cr1 deficiency exacerbated tau pathology and led to cognitive impairment. However, it is still unclear if the chemokine domain of the
Rosa Maisto et al.
Frontiers in pharmacology, 11, 235-235 (2020-03-27)
Stimulation of retinal photoreceptors with elevated glucose concentration (30 mM) for 96 h, served as diabetic retinopathy in vitro model to study Resolvin D1 (50 nM) effects on neovascularization. VEGF and anti-angiogenic miR-20a-3p, miR-20a-5p, miR-106a-5p, and miR-20b expression was assessed
查看所有相关文献

商品

荧光标记抗体_荧光WesternBlot-默克生命科学

荧光Western blot检测因具有多重检测的方便性而变得越来越受研究者的欢迎，但这种检测仍需要专门优化的工具来获得理想的实验结果。这里我们推荐一个最佳组合，以帮助大家在荧光检测中快速获得稳定、易重复的数据。

相关内容

User Guide - Immobilon® FL Transfer Membrane
Low Background Membrane for Fluorescent Protein Detection in Western Blotting
Immobilon®-E Transfer Membrane User Guide
Western Blotting Tools

Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.

查看所有结果

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持