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Merck
CN

IPVH85R

Millipore

Immobilon®-P Membrane

1 roll, 8.5 cm x 10 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

别名:

Immobilon® NOW, Immobilon® narrow roll, Western blotting membrane, blotting membrane, transfer membrane

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关于此项目

UNSPSC代码:
41105339
eCl@ss:
32031613
NACRES:
NB.23

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Product Name

Immobilon®-P膜，PVDF，0.45 m, 8.5 cm x 10 m卷, 0.45 um ( 0.45 micron filter ) pore size Hydrophobic immobilon-P PVDF Transfer Membrane for western blotting using Chemiluminescence, Chromogenic or Radioactive detection methods. Comes in an 8.5 cm x 10 m roll.

物料

PVDF membrane
plain filter
white filter

质量水平

400

特点

hydrophobic

制造商/商品名称

Immobilon®

技术

dot blot: suitable
western blot: suitable

过滤器长度 × 宽度

8.5 cm × 10 m

孔径

0.45 μm pore size

容量

160 μg/cm2 adsorption capacity (insulin)
 215 μg/cm2 adsorption capacity (BSA)
 294 μg/cm2 adsorption capacity (goat IgG)

相容性

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination

检测方法

chemiluminescent
colorimetric
fluorometric
radioactive

运输

ambient

相关类别

一般描述

Immobilon®-P转移膜是一种疏水的聚偏氟乙烯(PVDF)微孔膜，标称孔径为0.45 μm。它被用于从各种凝胶基质转移蛋白质。Immobilon®-P转移膜推荐用于大多数蛋白质印迹应用，尤其是大于20 kDa的蛋白质。与硝酸纤维素膜相比，该膜具有改善的处理特性和染色能力，更高信噪比，改善的灵敏度，以及改善的耐溶剂性。
主要结合机制：静电，疏水
过滤器代码：IPVH

应用

Immobilon®-P 膜，PVDF, 0.45 μm, 8.5 cm x 10 m 卷已被用于蛋白质印迹分析 和免疫共沉淀。

其他说明

替代品：INCP00010

法律信息

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

分析证书(COA)

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Chunzhi Li et al.
Investigative ophthalmology & visual science, 61(10), 9-9 (2020-08-07)
Retinoblastoma (RB) is the most common primary intraocular tumor in children. Chemoresistance is the major obstacle for treatment of these tumors. This study aims to determine whether or not downregulating microRNA-222 (miR-222) could serve as a potential therapeutic target for
Wei Cai et al.
Gene, 736, 144386-144386 (2020-01-25)
Neuropathic pain, caused by damage to the nerve system, is one of the most challenging diseases in the world. Moreover, the etiology remains unclear. Circular RNAs (circRNAs) have been revealed to participate in various biological progress, including neuropathic pain. However
microRNA-222-Mediated VHL Downregulation Facilitates Retinoblastoma Chemoresistance by Increasing HIF1? Expression.
Chunzhi Li, et al.
Investigative Ophthalmology & Visual Science (2020)
Jeremiah Tsyporin et al.
Cell reports, 35(12), 109269-109269 (2021-06-24)
Projection neuron subtype identities in the cerebral cortex are established by expressing pan-cortical and subtype-specific effector genes that execute terminal differentiation programs bestowing neurons with a glutamatergic neuron phenotype and subtype-specific morphology, physiology, and axonal projections. Whether pan-cortical glutamatergic and

商品

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

实验方案

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

免疫印迹膜剥离和再生检测

本页内容介绍和讨论了三种免疫印迹膜剥离和重新检测的方案。

WB检测样本前处理：细胞裂解和蛋白提取

Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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