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ISEQ00010

Immobilon® -PSQ PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.2 µm pore size, transfer membrane for Western blotting

别名:

Western blotting membrane, blotting membrane, transfer membrane

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关于此项目

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.23
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, radioactive
Pore size:
0.2 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination
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产品名称

Immobilon®-PSQ PVDF膜, 1 roll, 27 cm x 3.75 m, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Quality Segment

400

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

27 cm × 3.75 m

pore size

0.2 μm pore size

capacity

262 μg/cm2 adsorption capacity (insulin), 340 μg/cm2 adsorption capacity (BSA), 448 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

detection method

chemiluminescent, colorimetric, radioactive

shipped in

ambient

General description

Immobilon®-PSQ膜是由聚偏氟乙烯(PVDF)制成的0.2 μm微孔膜。该产品是为了最大化蛋白质结合而开发的。Immobilon®-PSQ膜推荐用于电泳凝胶电泳蛋白（或纯化蛋白样品的点结合）后的直接蛋白测序和免疫检测，特别是分子量小于20,000的蛋白。该滤膜兼容储水罐和半干法电印迹系统。

Application

Immobilon®-PSQ PVDF膜已用于蛋白质印迹分析。

Features and Benefits

Immobilon®-PSQ膜：
  • 0.2 μm孔径和大内部结构
  • 蛋白质吸附和测序产量高于其他膜
  • 防止低分子量蛋白质的吹穿

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Still not finding the right product?

Explore all of our products under Immobilon®-PSQ PVDF膜

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

分析证书(COA)

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实验方案

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

WB检测样本前处理：细胞裂解和蛋白提取

Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。

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相关内容

User Guide - Immobilon® PSQ Transfer Membrane
History of Western Blotting

"Timeline of western blotting history. Timeless recipes and solutions for blotting success. Prepare and grow cell, media and sera with cultureware. Filter media with Stericup. ProteoExtract® kits for fractionation. CytoBuster™, BugBuster™ and CelLytic™ protein extraction reagents. Benzonase® nuclease. cOmplete™ and SIGMAFAST™ protease inhibitorsMaintain and preserve protein functionality following cell lysis using individual protease inhibitors and protease inhibitor cocktails. Amicon® filters for concentration and buffer exchange. PureProteome® magnetic beads for enrichment and purification. Recombinant protein purification affinity gels. Unlike methods that require careful aspiration to avoid sample loss, PureProteome® magnetic beads are isolated using a magnetic rack, for total removal of buffers and complete recovery with no sample dilution. TruPAGE™ precast gels Buffers (including Trizma®Tris base), solvents, prestained MW markers. Direct Detect® IR spectrometer for protein sample qualification. TruPAGE® precast gels are tear-resistant to reduce handling failures that commonly occur during gel electrophoresis and downstream processing. Range of Immobilon® PVDF transfer membranes. Western-validated antibodies. SNAP i.d.® 2.0 protein detection system. Immunodetection reagents. Blot efficiently using our Western blotting reagents, including Bløk® noise-cancelling reagents, Luminata™ Western HRP substrates, Immunoreaction Enhancer Reagents, and ReBlot™ Plus antibody stripping kits, all carefully optimized for your success."

Immobilon®-PSQ Transfer Membrane User Guide
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全球贸易项目编号

货号GTIN
ISEQ0001004053252334306