Merck
CN
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文件

ISEQ00010

Millipore

Immobilon®-PSQ PVDF膜

1 roll, 27 cm x 3.75 m, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

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别名:
Western blotting membrane, blotting membrane, transfer membrane
eCl@ss:
32031602

物料

PVDF membrane
plain filter
white filter

质量水平

400

特点

hydrophobic

制造商/商品名称

Immobilon®

技术

dot blot: suitable
western blot: suitable

过滤器长度 × 宽度

27 cm × 3.75 m

孔径

0.2 μm pore size

容量

262 μg/cm2 adsorption capacity (insulin)
 340 μg/cm2 adsorption capacity (BSA)
 448 μg/cm2 adsorption capacity (goat IgG)

相容性

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination

检测方法

chemiluminescent
colorimetric
radioactive

运输

ambient

相关类别

一般描述

Immobilon®-PSQ膜是由聚偏氟乙烯(PVDF)制成的0.2 μm微孔膜。该产品是为了最大化蛋白质结合而开发的。Immobilon®-PSQ膜推荐用于电泳凝胶电泳蛋白（或纯化蛋白样品的点结合）后的直接蛋白测序和免疫检测，特别是分子量小于20,000的蛋白。该滤膜兼容储水罐和半干法电印迹系统。

应用

Immobilon®-PSQ PVDF膜已用于蛋白质印迹分析。

特点和优势

Immobilon®-PSQ膜：
  • 0.2 μm孔径和大内部结构
  • 蛋白质吸附和测序产量高于其他膜
  • 防止低分子量蛋白质的吹穿

法律信息

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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实验方案

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

免疫印迹膜剥离和再生检测

本页内容介绍和讨论了三种免疫印迹膜剥离和重新检测的方案。

WB检测样本前处理：细胞裂解和蛋白提取

Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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