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Merck
CN

ISEQ07850

Immobilon® -PSQ PVDF Membrane

50 sheets, 7 cm x 8.4 cm, 0.2 µm pore size, transfer membrane for Western blotting

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UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric, radioactive
Pore size:
0.2 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Toluidine blue, for use with Transillumination
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产品名称

Immobilon®-PSQ PVDF膜, 50 sheets, 7 cm x 8.4 cm, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

7 cm × 8.4 cm

pore size

0.2 μm pore size

capacity

262 μg/cm2 adsorption capacity (insulin), 340 μg/cm2 adsorption capacity (BSA), 448 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Toluidine blue, for use with Transillumination

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

shipped in

ambient

Quality Level

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General description

Immobilon®-PSQ转印膜是由聚偏氟乙烯(PVDF)制成的0.2 μm微孔膜,特别开发用于最大化蛋白吸附能力。这种转印膜适合用于凝胶电泳蛋白电印迹(或纯化蛋白样品的印迹吸附)后的直接蛋白测序和免疫检测。Immobilon®-PSQ转印膜为分子量小于20 kDa的蛋白质提供了最佳印迹。它的比表面积更大,对小分子蛋白的吸附能力更强。
过滤器编码:ISEQ
主要吸附机制:静电,疏水

Application

Immobilon-PSQ PVDF转印膜已用于蛋白质印迹分析(western blotting)。

Features and Benefits

Immobilon-PSQ转印膜:
  • 适合罐式和半干式电印迹系统
  • 蛋白质吸附和测序产量高于其他膜
  • 可防止低分子量蛋白质穿膜(blow-through)

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

10-13 - German Storage Class 10 to 13


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实验方案

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明

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There’s so much room for experimental variability in traditional immunodetection workflows. For your peace of mind – and ours – we designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry experiments. The concept is simple: a vacuum-driven flow of blocking, antibody, and washing solutions reduces slide and membrane handling. That means a lot less shaking, dipping, pouring and waiting. And now you can process multiple blots and slides in parallel, so it’s easy to apply consistent conditions across experiments.

Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.

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