CellASIC ONIX哺乳动物细胞梯度板（4腔）

Convert your microscope to a live cell imaging system – the CellASIC® ONIX2 system allows precise manipulation of the cell culture environment for dynamic live cell imaging and analysis using mammalian, yeast, and bacterial cells.

Perturbation analysis of live neurons is crucial for fully understanding the nervous system and requires spatiotemporal microenvironment control of cultured neurons. Although advances in microfluidics have enabled such studies, most microfluidic analyses of neurons have required prohibitive commitment of resources. We have demonstrated a novel, optimized microfluidic platform for long-term culture of primary neurons to monitor dynamic cellular processes in real time. The platform enables users to program automated changes to culture conditions without interrupting live cell analysis experiments. In addition, the microfluidic plates offer a superior platform for visualization when compared to plasticware used for conventional static culture. Finally, the platform requires little to no specialized expertise or dedicated personnel for operation, thereby presenting significant advantages over traditional microfluidic cell culture systems.

We demonstrate here the ability to conduct gene expression analysis using the CellASIC™ ONIX System with M04S microfluidic plates. Ultimately, directly correlating gene expression patterns to phenotypes observed during live cell imaging can provide powerful, meaningful functional genomics data, revealing signaling networks and novel biomolecular interactions.