Anti-Erythropoietin Antibody, clone 1C8

34 kDa (glycosylated)

Erythropoietin (Epo) is a glycoprotein consisting of one 165-amino acid single-chain polypeptide with two internal disulfide bonds. When fully glycosylated, the molecular weight of Epo is approximately 34 kDa. Normally produced by the liver and kidney, Epo is the principle regulator of red blood cell production by stimulating the division and differentiation of bone marrow erythroid progenitor cells in response to anemia and hypoxia.

Recombinant human Epo.

Detect Erythropoietin using this Anti-Erythropoietin Antibody, clone 1C8 validated for use in ELISA, IF & WB.

ELISA:
For the determination of Epo concentration in the range of 2-200 ng/mL. Use at 5 ug/mL to coat plate.

Immunosorbent preparation

Optimal working dilutions must be determined by the end user.

Research Category
Metabolism

Research Sub Category
Metabolic Hormones & Receptors

Reacts with human Erythropoietin (Epo).

Format: Purified

Protein A purified

Purified mouse monoclonal IgG2b in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.

Stable for 1 year at 2-8ºC from date of receipt.

Control
Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Evaluated by Western Blot on Mouse kidney lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected glycosylated EPO on 10 µg of Mouse kidney lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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