Anti-MMP-13 Antibody, clone LIPCO IID1

Rat MMP-13 from BC-1 cells.

Anti-MMP-13 Antibody, clone LIPCO IID1 is an antibody against MMP-13 for use in WB.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Western Blotting (Lyons, 1991; Lyons, 1993): 1:200-1:400 at room temperature for 2 hours.

Immunofluorescence

Optimal working dilutions must be determined by end user.

It recognizes proteins of ~60 kDa and ~48 kDa which are identified as pro (latent) and activate forms of matrix metalloproteinase-13 (MMP-13; also known as Collagenase-3). Antibody shows no cross-reactivity with the pro and active forms of other MMPs. Human collagenase-3 (MMP13) is a recently identified member of the matrix metalloproteinase (MMP) family that is expressed in breast carcinomas and in articular cartilage from arthritic patients. The MMP-13 gene has been isolated and characterized. This gene is composed of 10 exons and 9 introns and spans over 12.5 kb. The overall organization of the collagenase-3 gene is similar to that of other MMP genes clustered at chromosome 11q22, including fibroblast collagenase (MMP-1), matrilysin (MMP-7), and macrophage metalloelastase (MMP-12), but is more distantly related to genes coding for stromelysin-3 (MMP-11), gelatinase-A (MMP-2), and gelatinase-B (MMP-9), which map outside of this gene cluster.

Cellular Localization:

Cytoplasmic

Format: Purified

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Control
POSITIVE CONTROL: Conditioned, serum-free medium from (PMA-treated) rat fibrosarcoma cells or rat keratinocyte CCL-4.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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