产品名称
Anti-TIMP-2 Antibody, clone 2TMP05, clone 2TMP05, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2TMP05, monoclonal
species reactivity
human, mouse, bovine, guinea pig, rabbit, rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TIMP2(7077)
Analysis Note
Control
POSITIVE CONTROL:
Pancreatic or colon carcinoma
POSITIVE CONTROL:
Pancreatic or colon carcinoma
Application
Detect TIMP-2 using this Anti-TIMP-2 Antibody, clone 2TMP05 validated for use in WB, IH(P).
Immunohistology (formalin/paraffin only): 2-4μg/ml 30 min at RT.
Western blotting: 1μg/ml for 2 hrs at RT.
(Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min. followed by cooling at RT for 20 min).
The optimal dilution for a specific application under a given set of experimental conditions should be determined by the investigator.
Western blotting: 1μg/ml for 2 hrs at RT.
(Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min. followed by cooling at RT for 20 min).
The optimal dilution for a specific application under a given set of experimental conditions should be determined by the investigator.
Research Category
Cell Structure
Cell Structure
Research Sub Category
MMPs & TIMPs
MMPs & TIMPs
Biochem/physiol Actions
MAB13446 recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinase-2 (TIMP-2). TIMP-2 is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. CHEMICON MAB13446 may be used to evaluate the invasive nature of some tumor types and determine the tissue distribution of TIMPs in other tumors such as adenomas and also its normal tissue.
CELLULAR LOCALIZATION:
Cytoplasmic
CELLULAR LOCALIZATION:
Cytoplasmic
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
A synthetic peptide from the N-terminal region of human TIMP-2 protein.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB13448
Physical form
Format: Purified
Purified from ascites fluid by Protein A chromatography. 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Preparation Note
Antibody with sodium azide is stable for 12 months after date of receipt when stored at 2-8°C.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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