Recombinant cPLA2 expressed from the human cPLA2 gene.

Detect Phospholipase A2 using this Anti-Phospholipase A2 Antibody, clone CH-7 validated for use in ELISA & WB.

Immunoblot: 1:500-1:1000; antibody recognizes all isoforms, the cPLA.

Alpha (85kDa) typically migrates as 100-110kDa on reducing westerns; beta migrates as 114kDa; antibody will also recognize the sPLA at approximately 14-20kDa.

ELISA

Optimal working dilutions must be determined by the end user.

Research Category
Signaling

Research Sub Category
Lipid Signaling

Antibody reacts with cytosolic form of PLA2. Antibody will also react with beta isoform. Epitope has been localized within the first 216 aa of the N-terminal Ca++ binding domain of cPLA2

Format: Purified

In 20 mM sodium phosphate, 250 mM sodium chloride, 0.1% sodium azide, pH. 7.6

Maintain at 2-8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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