产品名称
抗ADAMTS-1抗体，克隆5D4E11B6, serum, clone 5D4E11B6, Chemicon®
biological source
mouse
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
5D4E11B6, monoclonal
species reactivity
human, mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ADAMTS1(9510)
相关类别
Analysis Note
对照
A431细胞裂解物
HEK293细胞裂解物
PC-12细胞裂解物
胚胎鼠脑裂解物
MCF-7细胞裂解物
A431细胞裂解物
HEK293细胞裂解物
PC-12细胞裂解物
胚胎鼠脑裂解物
MCF-7细胞裂解物
Application
使用经验证可用于ELISA、IP& WB的抗ADAMTS-1抗体（克隆5D4E11B6）检测ADAMTS-1。
蛋白质印迹： 在A431细胞裂解物上以1：2000-1：4000稀释：
注： 检测到的MW条带因检测酶原和/或活性形式的细胞系或样品而异。
免疫沉淀： 使用克隆5D4E11B6通过免疫印迹确认；使用克隆5C6D5（目录号MAB1771）以1：50免疫沉淀500µg HEK293全细胞裂解物或PC-12全细胞裂解物中的ADAMTS-1
注： 检测到的MW条带因检测酶原和/或活性形式的细胞系或样品而异。
免疫沉淀： 使用克隆5D4E11B6通过免疫印迹确认；使用克隆5C6D5（目录号MAB1771）以1：50免疫沉淀500µg HEK293全细胞裂解物或PC-12全细胞裂解物中的ADAMTS-1
Biochem/physiol Actions
与其他物种的反应性尚未检验。
克隆5D4E11B6可识别人ADAMTS-1。
General description
110 / 87 / 65 kDa
ADAMTS-1是称为ADAMTS（具有血小板反应蛋白基序的解整联蛋白和金属蛋白酶）的细胞外蛋白酶家族的成员。ADAMTS家族的成员共享不同的蛋白模块，包括前肽区域，金属蛋白酶结构域，解整联蛋白样结构域和血小板反应蛋白1型（TSP）基序。该家族的各个成员在C末端TSP基序的数量上有所不同，并且有些具有独特的C末端结构域。该基因编码的蛋白包含两个解整联蛋白环和三个C端TSP基序。ADAMTS-1裂解软骨蛋白聚糖聚集蛋白聚糖，并似乎显示出血管生成抑制活性。ADAMTS-1的成熟需要两个独立且连续的加工事件，这些事件可能会释放两种形式的蛋白。随着从110 kDa酶原中切割前结构域，保留了87 kDa的活性形式，可以通过从TSP重复序列中去除催化亚基来进一步加工，留下C端65 kDa的可溶形式。最后两个TSP重复序列的蛋白水解缺失可能对该蛋白的体内功能很重要。
Immunogen
基于人序列的Myc-His羧基标记的全长重组蛋白。
Physical form
腹水。含0.05%NaN3的液体。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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