生物来源
rat
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
YL 1/2, monoclonal
种属反应性
yeast, porcine, rat, chicken, bovine, human, mouse
技术
ELISA: suitable
electron microscopy: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable
western blot: suitable
同位素/亚型
IgG2aκ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... TUBA1A(7846)
一般描述
免疫原
应用
粘附(CAMs)
细胞结构
免疫细胞化学分析:4.0µ g/mL代表性批次在A431、HeLa和HUVEC细胞中检测到酪氨酸化α-微管蛋白。
免疫细胞化学分析:代表性批次在酵母中检测到苯丙氨酸化α-微管蛋白的免疫反应性,在HEK293细胞中检测到酪氨酸化α-微管蛋白的免疫反应性(Kilmartin J.V., et al. (1982).J Cell Biol. 93(3):576-582.;Bandyopadhyay, B., et al. (2007).J. Biol. Chem. 282(22):16454-16464)。
免疫组化分析:代表性批次在Hamburger-Hamilton阶段4(HH4)雏鸡胚胎的中线水平切片中检测到微管酪氨酸化α-微管蛋白免疫反应性(Nakaya, Y., et al. (2008).Nat. Cell Biol. 10(7):765-775)。
电子显微镜分析:代表性批次使用粗酵母核被膜制剂检测到微管苯丙氨酸化的α-微管蛋白免疫反应性,并在NIH/3T3细胞中检测到微管酪氨酸化的α-微管蛋白免疫反应性(Wehland, J., and Willingham, M.C.(1983).J Cell Biol. 97(5 Pt 1):1476-1490; Kilmartin J.V., et al. (1982).J Cell Biol. 93(3):576-582)。
ELISA分析:使用猪脑源性微管蛋白对在C-末端有或没有Tyr/Phe的α-微管蛋白肽,通过竞争性ELISA测定克隆YL1/2的抗原性(Wehland, J., et al. (1984).EMBO J. 3(6):1295-1300)。
放射免疫测定法分析:通过放射免疫测定法测定克隆YL1/2对鸡脑微管蛋白和酵母微管蛋白的免疫反应性(Kilmartin J.V., et al. (1982).J Cell Biol. 93(3):576-582)。
生化/生理作用
外形
制备说明
分析说明
蛋白质印迹分析:1.0 µg/mL 的该抗体在 10 µg A431 细胞裂解物中检测到酪氨酸化的 α-微管蛋白。
其他说明
免责声明
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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