产品名称
Anti-Laminin α2 Antibody, clone 5H2, Azide Free, clone 5H2, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
5H2, monoclonal
species reactivity
human, rabbit, monkey
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... LAMA2(3908)
rhesus monkey ... Lama2(715394)
Application
Anti-Laminin α2 Antibody, clone 5H2, Azide Free detects level of Laminin α2 & has been published & validated for use in ELISA, IH, IP & WB.
ELISA: 50% maximal binding to human merosin at 1:50,000.
Immunohistochemistry: 1:5,000 for staining of 8 mm acetone-fixed cryostat muscle sections, prior to detection with a peroxidase-conjugated secondary antibody.
Immunofluorescence
Affinity chromatography
Immunoprecipitation
Immunoblotting
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 1:5,000 for staining of 8 mm acetone-fixed cryostat muscle sections, prior to detection with a peroxidase-conjugated secondary antibody.
Immunofluorescence
Affinity chromatography
Immunoprecipitation
Immunoblotting
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Biochem/physiol Actions
Reacts with the 80 kDa fragment of the M-chain of human merosin.
SPECIES REACTIVITIES:
Cross reacts with monkey and rabbit merosin.
SPECIES REACTIVITIES:
Cross reacts with monkey and rabbit merosin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Purified human merosin.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Preparation Note
Maintain between 2 and 8°C for up to 6 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Corinna Preuße et al.
Acta neuropathologica communications, 4(1), 45-45 (2016-04-29)
Dermatomyositis (DM) can occur in both adults and juveniles with considerable clinical differences. The links between immune-mediated mechanisms and vasculopathy with respect to development of perifascicular pathology are incompletely understood. We investigated skeletal muscle from newly diagnosed, treatment-naïve juvenile (jDM)
Elizabeth I Tang et al.
Endocrinology, 163(11) (2022-08-17)
Microtubule affinity-regulating kinases (MARKs) are nonreceptor Ser/Thr protein kinases known to regulate cell polarity and microtubule dynamics in Caenorhabditis elegans, Drosophila, invertebrates, vertebrates, and mammals. An earlier study has shown that MARK4 is present at the ectoplasmic specialization and blood-testis
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