MAB19292
抗-ADAM2抗体，克隆9D2.2
clone 9D2.2, Chemicon®, from mouse
别名:
Fertilin beta
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
9D2.2, monoclonal
Application:
ELISA, WB
Species reactivity:
mouse
Citations:
15
Technique(s):
ELISA: suitable, western blot: suitable
Uniprot accession no.:
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
9D2.2, monoclonal
species reactivity
mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, western blot: suitable
isotype
IgG2b
suitability
not suitable for immunohistochemistry
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ADAM2(2515)
Application
研究子类别
ECM蛋白
MMP & TIMP
ECM蛋白
MMP & TIMP
研究类别
细胞结构
细胞结构
蛋白质印迹：1:500 - 1:1,000稀释液可检测精子裂解物，使用标准碱性磷酸酶与NBT/BCIP检测。 化学发光检测允许更高的稀释度。
对免疫组织化学无效。
最佳工作稀释度必须由最终用户进行确定。
对免疫组织化学无效。
最佳工作稀释度必须由最终用户进行确定。
该抗-ADAM2抗体，克隆9D2.2，经验证可用于ELISA、WB中ADAM2的检测。
Biochem/physiol Actions
通过免疫印迹识别小鼠受精蛋白β，在46-48 kDa范围内进行条带染色（多条带是由于可变蛋白质糖基化）。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Immunogen
通过在SF9细胞中表达杆状病毒产生重组小鼠受精蛋白。
Physical form
形式：纯化
液体形式，1 mg/mL，溶于0.02M磷酸盐缓冲液，pH 7.6，含0.25M NaCl和0.1%叠氮化钠。
Preparation Note
以未稀释的等分形式可在2-8°C冷藏保存长达12个月。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Daiji Kiyozumi et al.
Biology of reproduction, 109(4), 474-481 (2023-08-02)
The mammalian epididymis is the organ for functional sperm maturation. In rodents, the initial segment, the most proximal region of the epididymis, plays a critical role in sperm maturation. The luminal epithelial differentiation and the following gene expression of the
Ryo Yamaguchi et al.
Biology of reproduction, 75(5), 760-766 (2006-07-28)
Male mice deficient for the calmegin (Clgn) or the angiotensin-converting enzyme (Ace) gene show impaired sperm migration into the oviduct and loss of sperm-zona pellucida binding ability in vitro. Since CLGN is a molecular chaperone for membrane transport of target
Kathryn K Stein et al.
Biology of reproduction, 73(5), 1032-1038 (2005-07-15)
Adam2-null and Adam3-null male mice exhibit reduced levels of one or more ADAM proteins on mature sperm, in addition to the loss of the genetically targeted protein. ADAM protein loss was believed to occur posttranslationally, although the timing of loss
Ryo Yamaguchi et al.
Genes to cells : devoted to molecular & cellular mechanisms, 13(8), 851-861 (2008-09-11)
CD52 is a glycosylphosphatidylinositol (GPI)-anchored antigen expressed on lymphocytes and in epididymal epithelial cells. CD52 is also known as "maturation-associated sperm antigen" but its function is unknown. We therefore generated Cd52 disrupted mice. The resulting Cd52 null mice were healthy
Yasutaka Ueda et al.
The Journal of biological chemistry, 282(42), 30373-30380 (2007-08-23)
A palmitate linked to the inositol in glycosylphosphatidylinositol (GPI) is removed in the endoplasmic reticulum immediately after the conjugation of GPI with proteins in most cells. Previously, we identified PGAP1 (post GPI attachment to proteins 1) as a GPI inositoldeacylase
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