MAB1973Z
Anti-Integrin α1 Antibody, clone FB12, azide free
clone FB12, Chemicon®, from mouse
别名:
CD49a, MAB1973
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
FB12, monoclonal
Application:
ELISA, FACS, IHC, IP, WB
Citations:
22
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
FB12, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ITGA1(3672)
General description
130 kDa
Application
Detect Integrin α1 using this Anti-Integrin α1 Antibody, clone FB12, azide free validated for use in ELISA, FC, IH, IP, FUNC & WB.
Immunohistochemistry: 2 μg/mL, with paraformaldehyde fixation. Immunoblotting: 2 μg/mL
Immunoprecipitation: 1 μg/mL
FACS analysis: 2 μg/mL
ELISA: 0.5 μg/mL
Inhibition of activated human lymphocyte binding to laminin, collagen IV and fibronectin (Fabbri, 1996): 1-5 μg/mL
Optimal working dilutions must be determined by end user.
Immunoprecipitation: 1 μg/mL
FACS analysis: 2 μg/mL
ELISA: 0.5 μg/mL
Inhibition of activated human lymphocyte binding to laminin, collagen IV and fibronectin (Fabbri, 1996): 1-5 μg/mL
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Reacts with the I domain (Val151 - Ala364) of human alpha1 integrin (Fabbri, 1996). Precipitates proteins of Mr 180 kDa, pI 5.9-6.0 (alpha chain) and Mr 115 kDa, pI 4.5-5.0 (beta chain) from human lymphocyte lysates (Zocchi, 1991). Does not recognize recombinant I domain of rat integrin alpha1.
Physical form
Format: Purified
Purified immunoglobulin in 0.02M PB, pH 7.6, 0.25M NaCl.
Analysis Note
Control
Microvascular endothelium
Microvascular endothelium
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable