产品名称
抗硫酸软骨素4抗体,克隆BE-123, ascites fluid, clone BE-123, Chemicon®
biological source
mouse
antibody form
ascites fluid
antibody product type
primary antibodies
clone
BE-123, monoclonal
species reactivity
human, canine, rat, sheep, bovine, mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
radioimmunoassay: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CSPG4(1464)
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Application
1:400-1:3,200的ELISA。
1:50-1:6,400的RIA。
免疫组化:该抗体仅与消化的物质反应。固定的组织必须用软骨素酶ABC(Sigma)消化。
IHC应标明参考号23757342。(Reine,TM.,et al.,PubMed ID:23757342)
蛋白质印迹:1:1000-1:4000采用ECL检测法。裂解物样品的软骨素酶ABC治疗有帮助。
在任何碱缓冲液中,例如Tris或乙酸盐(50mM或100mM)pH值7.4-7.6。 通过在100 µL中稀释一瓶Sigma软骨素酶A、B、C(5单位/瓶)制备贮备液。 使用时,将贮备液稀释10倍至所选缓冲液中。 以每5单位酶含蛋白聚糖50~100mg的比例使用。 在固定细胞上,用三羟甲基氨基甲烷稀醋酸盐释,在非固定细胞上,用DMEM稀释10-25倍。
Poole,C.A.,et al.,J. Histochem.Cytochem.1991. 39:1175-1187。
最佳工作稀释度必须由最终用户确定。
1:50-1:6,400的RIA。
免疫组化:该抗体仅与消化的物质反应。固定的组织必须用软骨素酶ABC(Sigma)消化。
IHC应标明参考号23757342。(Reine,TM.,et al.,PubMed ID:23757342)
蛋白质印迹:1:1000-1:4000采用ECL检测法。裂解物样品的软骨素酶ABC治疗有帮助。
在任何碱缓冲液中,例如Tris或乙酸盐(50mM或100mM)pH值7.4-7.6。 通过在100 µL中稀释一瓶Sigma软骨素酶A、B、C(5单位/瓶)制备贮备液。 使用时,将贮备液稀释10倍至所选缓冲液中。 以每5单位酶含蛋白聚糖50~100mg的比例使用。 在固定细胞上,用三羟甲基氨基甲烷稀醋酸盐释,在非固定细胞上,用DMEM稀释10-25倍。
Poole,C.A.,et al.,J. Histochem.Cytochem.1991. 39:1175-1187。
最佳工作稀释度必须由最终用户确定。
已发表的抗硫酸软骨素4抗体,克隆BE-123可检测硫酸软骨素4&水平&经验证可用于ELISA、RIA &WB。
研究子类别
ECM 蛋白
ECM 蛋白
研究类别
细胞结构
细胞结构
Biochem/physiol Actions
与小鼠(胎儿和成年)和人(胎儿和成人)软骨素酶ABC消化的软骨蛋白聚糖反应。 硫酸软骨素-4二糖成功地抑制了单克隆抗体与蛋白聚糖的结合。 与天然蛋白聚糖或软骨素酶AC或睾丸透明质酸酶消化的蛋白聚糖无反应。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Immunogen
小鼠蛋白聚糖。
Physical form
不含防腐剂的腹水。
Preparation Note
收到货后,在-20°C下以未稀释等分试样可保存长达12个月。 应避免反复冻/融循环。
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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