产品名称
Anti-Disialoganglioside GD3 Antibody, clone MB3.6, clone MB3.6, Chemicon®, from mouse
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
MB3.6, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
isotype
IgG3
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Application
Anti-Disialoganglioside GD3 Antibody, clone MB3.6 is an antibody against Disialoganglioside GD3 for use in FC, IF, IH.
Immunohistochemistry (frozen tissue sections)
Immunofluorescence of cells in culture
Flow cytometry: 1-2 μg per 10E6 cells
Lysis of GD3 positive (melanoma) cells in presence of complement (Cheresh et al., 1985).
Induction of antibody dependent cellular cyotoxicity (ADCC) {Ortaldo JR et al 1987 J Immunol 138(10):3566-3572.
TLC analysis: Sjoberg, ER (1995) J. Biol. Chem. 270(7):2921-2930.{http://www.jbc.org/cgi/content/full/270/7/2921}
Optimal working dilutions must be determined by end user.
Immunofluorescence of cells in culture
Flow cytometry: 1-2 μg per 10E6 cells
Lysis of GD3 positive (melanoma) cells in presence of complement (Cheresh et al., 1985).
Induction of antibody dependent cellular cyotoxicity (ADCC) {Ortaldo JR et al 1987 J Immunol 138(10):3566-3572.
TLC analysis: Sjoberg, ER (1995) J. Biol. Chem. 270(7):2921-2930.{http://www.jbc.org/cgi/content/full/270/7/2921}
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Reacts specifically with human GD3 ganglioside (Cheresh et al., 1984). No cross-reactivity with other gangliosides purified from neuroblastoma or melanoma cells.
Immunogen
FM9 melanoma cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% NaN3.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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