产品名称
Anti-Integrin α3 Antibody, clone ASC-1, clone ASC-1, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
ASC-1, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ITGA3(3675)
Application
Anti-Integrin α3 Antibody, clone ASC-1 detects level of Integrin α3 & has been published & validated for use in FC, IP, IH.
Inhibition of cell attachment: inhibits SCC9 cell adhesion to human and
EHS laminin, and laminin GD-2 peptide. Potentiates inhibition of SKOV-3 cell adhesion to EHS laminin by anti-integrin alpha 5 antibody GoH3. Does not inhibit cell adhesion to fibronectin or collagen type IV.
Immunohistochemistry: on acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue.
Flow cytometry: stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelial cells (HUVEC).
Immunoprecipitation: precipitates PAGE bands of 130kDa (reduced) or 116kDa and 135kDa (non-reduced) from surface-biotinylated SCC9 or SKOV-3 cells.
Working dilutions must be determined by end user.
EHS laminin, and laminin GD-2 peptide. Potentiates inhibition of SKOV-3 cell adhesion to EHS laminin by anti-integrin alpha 5 antibody GoH3. Does not inhibit cell adhesion to fibronectin or collagen type IV.
Immunohistochemistry: on acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue.
Flow cytometry: stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelial cells (HUVEC).
Immunoprecipitation: precipitates PAGE bands of 130kDa (reduced) or 116kDa and 135kDa (non-reduced) from surface-biotinylated SCC9 or SKOV-3 cells.
Working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
Biochem/physiol Actions
Human alpha3 integrin (CD49c)
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Human squamous cell carcinoma cells (SCC9)
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: CBL462B
Physical form
Format: Purified
Liquid in 0.02M phosphate buffer, pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 12 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Edith Hintermann et al.
The Journal of cell biology, 153(3), 465-478 (2001-05-02)
Keratinocytes and other epithelial cells express two receptors for the basement membrane (BM) extracellular matrix component laminin-5 (Ln-5), integrins alpha 3 beta 1 and alpha 6 beta 4. While alpha 3 beta 1 mediates adhesion, spreading, and migration (Kreidberg, J.A.
Julia K L Woodward et al.
Investigative ophthalmology & visual science, 46(10), 3473-3477 (2005-09-28)
TGFbeta has been shown to have a regulatory effect on uveal melanoma invasion, but it is not known which processes are specifically influenced. The purpose of this study was to analyze the effect of TGFbeta stimulation on the adhesive interactions
M Shang et al.
The Journal of biological chemistry, 276(35), 33045-33053 (2001-06-08)
Laminins are a family of extracellular matrix glycoproteins involved in cell adhesion and migration. A major obstacle to understanding their structure-function relationships is the lack of small laminin domains capable of replicating integrin-binding, cell-adhesive, and migratory functions of the intact
Paul Day et al.
International journal of oncology, 39(4), 863-871 (2011-07-02)
Integrins, a family of heterodimeric receptors for cell adhesion to the extracellular matrix (ECM), play key roles in cell migration, cancer progression and metastasis. As transmembrane proteins, integrins are transported in vesicles and delivered to the cell surface by vesicular
Expression of laminin isoforms, receptors, and binding proteins unique to nucleus pulposus cells of immature intervertebral disc.
Chen, J; Jing, L; Gilchrist, CL; Richardson, WJ; Fitch, RD; Setton, LA
Connective tissue research null
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