Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface, members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNF alpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NK kappa B dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

Human E-Selectin

IL-1(1 activated HUVEC (human)

Anti-E-Selectin Antibody, clone P2H3 detects level of E-Selectin & has been published & validated for use in ELISA, FC, IP, IC, IH.

Immunohistochemistry: 1:50 – 1:500; acetone or paraformaldehyde-fixed frozen and formalin-fixed paraffin-embedded tissues; recommend heat-induced epitope retrieval in EDTA, pH 8.0 for paraffin-embedded tissues
Immunofluorescence
Flow Cytometry
Immunoprecipitation
ELISA
Blocking: 10-15µg/mL; inhibits carcinoma and monocyte adhesion to IL-1β activated HUVEC

Optimal working dilutions must be determined by end user.

Format: Purified

Purified by protein G and stored in 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl containing 0.05% NaN₃.

Maintain at 2-8°C for up to 12 months from date of receipt..

Control
Ductal breast cancer or mormal colon

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany