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Merck
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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41
技术服务
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生物来源

mouse

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

P2H3, monoclonal

种属反应性

human

制造商/商品名称

Chemicon®

技术

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

同位素/亚型

IgG1

NCBI登记号

UniProt登记号

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... SELE(6401)

一般描述

Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface, members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNF alpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NK kappa B dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

特异性

Human E-Selectin

免疫原

IL-1(1 activated HUVEC (human)

应用

Anti-E-Selectin Antibody, clone P2H3 detects level of E-Selectin & has been published & validated for use in ELISA, FC, IP, IC, IH.
Immunohistochemistry: 1:50 – 1:500; acetone or paraformaldehyde-fixed frozen and formalin-fixed paraffin-embedded tissues; recommend heat-induced epitope retrieval in EDTA, pH 8.0 for paraffin-embedded tissues
Immunofluorescence
Flow Cytometry
Immunoprecipitation
ELISA
Blocking: 10-15µg/mL; inhibits carcinoma and monocyte adhesion to IL-1β activated HUVEC

Optimal working dilutions must be determined by end user.

外形

Format: Purified
Purified by protein G and stored in 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl containing 0.05% NaN3.

储存及稳定性

Maintain at 2-8°C for up to 12 months from date of receipt..

分析说明

Control
Ductal breast cancer or mormal colon

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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