生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
15D12.2, monoclonal
种属反应性
human, mouse
种属反应性(根据同源性预测)
rat (based on 100% sequence homology)
技术
immunohistochemistry: suitable
western blot: suitable
同位素/亚型
IgG2bκ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... TPM1(7168)
一般描述
Tropomyosins (Tms) are a family of >40 highly conserved protein isoforms resulting from alternative splicing of four different genes during development and that are expressed in numerous cell types. These proteins form coiled-coil heterodimers which bind to actin polymers along the α-helical grove. Tropomyosin’s function varies depending on cell type in which it is expressed. Within muscle, Tms play a role in the regulation of actin-myosin interplay for the purpose of mediating muscle contraction. In non-muscle cells, studies suggest a role in the stabilization of actin filaments, along with protecting actin filaments from the severing action of gelsolin and ADF/cofilin depolymerization. Tms also appear to have a role in the moderation of access to actin for other actin-binding proteins. Tropomyosin has an integral role in maintaining cardiovascular homeostasis. Down-regulation of Tms expression has been observed in tumor cells indicating a possible role for Tms as tumor suppressors.
~ 30-40 kDa observed MWs. Tm6: 40 kDa; Tm1: 38 kDa; Tm2: 36 kDa; Tm3: 34 kDa; Tm5: 30 kDa
特异性
The antibody recognizes the entire exon 9d of Tropomyosin.
免疫原
Epitope: Exon 9d
Linear peptide corresponding to the entire exon 9d of rat Tropomyosin.
应用
Detect Tropomyosin using this Anti-Tropomyosin Antibody, clone 15D12.2 validated for use in WB, IH.
Immunohistochemistry Analysis: 1:300 dilution from a representative lot detected Tropomyosin in human striated muscle tissue.
Research Category
Cell Structure
Metabolism
Cell Structure
Metabolism
Research Sub Category
Cytoskeleton
Muscle Physiology
Cytoskeleton
Muscle Physiology
外形
Protein G
Format: Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Mouse brain tissue lysate
Mouse brain tissue lysate
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: 0.5 µg/mL of the antibody detected Tropomyosin in 10 µg of mouse brain tissue lysate.
Western Blot Analysis: 0.5 µg/mL of the antibody detected Tropomyosin in 10 µg of mouse brain tissue lysate.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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