biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
9D7.2, monoclonal
species reactivity
rabbit, fish
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable, western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
rabbit ... Gjd2(100341036)
Immunogen
Recombinant Perch Connexin 35
Application
Anti-Connexin 35/36 Antibody, clone 9D7.2 detects level of Connexin 35/36 & has been published & validated for use in WB, IH.
Immunohistochemistry
Western blot: 1:500-1:1000
Optimal working dilutions must be determined by end user.
Western blot: 1:500-1:1000
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Biochem/physiol Actions
Recognizes Perch Connexin 35. Also recognizes rabbit Connexin 36, the gene homologue of Connexin 35. Labels gap junctions in retinas of perch and rabbit in immunohistochemistry.
Physical form
Format: Purified
Purified immunoglobulin. Liquid in 0.1M Citrate buffer with 50% glycerol and 0.1% Sodium Azide as a preservative.
Preparation Note
Maintain at -20°C for up to 12 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 2
John E Rash et al.
Neuron, 79(5), 957-969 (2013-09-10)
Electrical synapses are abundant in the vertebrate brain, but their functional and molecular complexities are still poorly understood. We report here that electrical synapses between auditory afferents and goldfish Mauthner cells are constructed by apposition of hemichannels formed by two
Trafficking of gap junction channels at a vertebrate electrical synapse in vivo.
Flores, CE; Nannapaneni, S; Davidson, KG; Yasumura, T; Bennett, MV; Rash, JE; Pereda, AE
Proceedings of the National Academy of Sciences of the USA null
Jose L Serrano-Velez et al.
Frontiers in neural circuits, 8, 66-66 (2014-07-16)
"Dye-coupling", whole-mount immunohistochemistry for gap junction channel protein connexin 35 (Cx35), and freeze-fracture replica immunogold labeling (FRIL) reveal an abundance of electrical synapses/gap junctions at glutamatergic mixed synapses in the 14th spinal segment that innervates the adult male gonopodium of
J E Rash et al.
Neuroscience, 285, 166-193 (2014-12-03)
Gap junctions provide for direct intercellular electrical and metabolic coupling. The abundance of gap junctions at "large myelinated club ending (LMCE)" synapses on Mauthner cells (M-cells) of the teleost brain provided a convenient model to correlate anatomical and physiological properties
Cong Yao et al.
Journal of neurophysiology, 112(9), 2102-2113 (2014-08-01)
In contrast to the knowledge of chemical synapses, little is known regarding the properties of gap junction-mediated electrical synapses in developing zebrafish, which provide a valuable model to study neural function at the systems level. Identifiable "mixed" (electrical and chemical)
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