MAB3083
Anti-Calpain Antibody, small subunit of µ- or m-Calpains (Calpain I or II), clone P1
ascites fluid, clone P1, Chemicon®
别名:
Anti-CALPAIN4, Anti-CANP, Anti-CANPS, Anti-CAPN4, Anti-CDPS, Anti-CSS1
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
P1, monoclonal
Application:
ELISA, ICC, IHC, WB
Citations:
12
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
P1, monoclonal
species reactivity
human, bovine
should not react with
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... CAPNS1(826)
General description
Calpains are non-lysosomal cysteine proteeases which absolutely require calcium for activity. There are two major calpain isozymes: μ-calpain and m-calpain, which require micromolar or millimolar calicum for activity, respectively, in invitro assays. The major calpain isozymes are composed of two subunits: an 80kDa catalytic subunit; and a 30kDa regulatory subunit. The large and small subunits remain associated in the presence of calcium after proteolytic activation of the zymogen.
Immunogen
Bovine myocardial m-calpain.
Epitope: small subunit of μ- or m-Calpains (Calpain I or II)
Application
Immunoblot: 1:500-1,000. The small subunit of either μ-calpain m-calpain is 30kDa. ELISA: 1:50,000 (against the immunogen). Immunohistochemistry: 1:100 on 4% paraformaldehyde or 1% paraformaldehyde/4% glutaraldehyde fixed tissue. Immunocytochemistry: 1:100. Optimal working dilutions must be determined by the end user.
This Anti-Calpain Antibody, small subunit of μ-or m-Calpains (Calpain I or II), clone P1 is validated for use in ELISA, WB, IC, IH for the detection of Calpain.
Biochem/physiol Actions
Reacts with the small subunit of human erthrocyte μ-calpain (Calpain I) or bovine myocardial m-calpain (Calpain II).
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable