MAB3083-C
Anti-Calpain, small subunit Antibody, clone P-1 (Ascites Free)
选择尺寸
关于此项目
conjugate
unconjugated
antibody form
purified antibody
clone
P1, monoclonal
purified by
using protein G
species reactivity
mouse, human, bovine
concentration
(Please refer to lot specific datasheet.)
technique(s)
activity assay: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1κ
UniProt accession no.
target post-translational modification
unmodified
Quality Level
General description
Application
Western Blotting Analysis: A representative lot detected steady level of cellular calpain small subunit during the final stage of human CD34+ erythroid progenitor cells differentiation to reticulocytes (Patterson, T., et al. (2009). J. Biol. Chem. 284(21):14547-1457).
Western Blotting Analysis: A representative lot detected upregulated calpain small subunit level in atrophied soleus muscle upon hind limbs immobilization by plaster cast among male rats (Sugiura, T., et al. (2003). J. Physiol. Sci. 56(6):393-399).
Western Blotting Analysis: A representative lot detected calpain small subunit in human WI-38 fibroblasts (Zhang, W., et al. (1996). J. Biol. Chem. 271(31):18825-18830).
Immunocytochemistry Analysis: A representative lot detected increrased plasma membrane calpain small subunit immunoreactivity following ionomcyin treatment of human large cell lung carcinoma LCLC-103H cells by fluorescent immunocytochemistry (Gil-Parrado, S., et al. (2003). J. Biol. Chem. 278(18):16336-16346).
Immunoprecipitation Analysis: Representative lots immunoprecipitated calpain small subunit from human WI-38 fibroblasts (Zhang, W., et al. (1996). J. Biol. Chem. 271(31):18825-18830; Zhang, W., and Mellgren, R.L. (1996). Biochem. Biophys. Res. Commun. 227(3):891-896.).
Activity Assay: A representative lot inhibited the proteolytic activity of bovine and canine calpain 2, as well as partially purified human erythrocyte calpain I as a result of increased Km and decreased Vmax toward the substrate casein (Mellgren, R.L., and Lane, R.D. (1988). Biochem. Biophys. Res. Commun. 954(2):154-160).
Biochem/physiol Actions
Physical form
Preparation Note
Analysis Note
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Calpain, small subunit in 10 µg of HEK293 cell lysate.
Other Notes
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Calpain, small subunit in human kidney, human testis, and human prostate tissue.
Disclaimer
存储类别
10 - Combustible liquids
法规信息
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