产品名称
Anti-TIMP-1 Antibody, clone 7-6C1, clone 7-6C1, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
7-6C1, monoclonal
species reactivity
rat, human, bovine
should not react with
mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TIMP1(7076)
Application
Detect TIMP-1 using this Anti-TIMP-1 Antibody, clone 7-6C1 validated for use in ELISA, WB, IH.
Immunoblotting: 1:1000, identifies a band of approximately 30KDa. Note TIMP-1 is found in very low concentrations in most cells and tissues.Positive control is HT1080 cells treated with PMA or recombinant TIMP-1 protein CC3328.
Immunohistochemistry on frozen sections or acetone fixed tissues. Formalin fixed paraffin-embedded tissue sections are usually not reactive. PLP fixation is suggested as an alternative.
EIA
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on frozen sections or acetone fixed tissues. Formalin fixed paraffin-embedded tissue sections are usually not reactive. PLP fixation is suggested as an alternative.
EIA
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
MMPs & TIMPs
MMPs & TIMPs
Biochem/physiol Actions
MAB3300 is a purified mouse monoclonal antibody to bovine tissue inhibitor of metalloproteinases-1 (bovine TIMP-1). The antibody specifically reacts with bovine TIMP-1 and cross-reacts with human and rat TIMP-1, but not with mouse TIMP-1. By western blot the antibody recognizes a glycoprotein of 28.5 kDa, which is identified as tissue inhibitor of metalloproteinases-1 (TIMP-1).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Tissue Inhibitors of Metalloproteinases (TIMPs) inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP-1 and TIMP-2 have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72 kDa type IV collagenase (also known as MMP-2 or gelatinase A), and latent and active forms 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-1 levels in quiescent cells and tissues is low (pg/ml), and stimulation with the phorbol ester TPA or protein concentration is often needed to visualize protein bands. In addition, cell types differ greatly in the amount of TIMP-1 produced.
Immunogen
Bovine TIMP-1
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1133
Physical form
Format: Purified
Purified immunoglobulin presented as a liquid in 0.1 M Na-Phosphate buffer, pH 7.0 containing protease free bovine serum albumin.
Preparation Note
Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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