产品名称
抗-绿色荧光蛋白抗体, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
target post-translational modification
unmodified
Application
使用经验证可用于ELISA、IC、IH & WB的抗绿色荧光蛋白抗体检测绿色荧光蛋白。
蛋白质印迹：1:2500-1:5000。建议的稀释缓冲液是含有1 mg/mL BSA的PBS。
免疫细胞化学：1:500-1:1000
免疫组织化学：在多聚甲醛固定的组织上以1:500-1:1,000的比例孵育过夜，使用镍增强的DAB进行检测。建议的透化方法是0.5% Triton X-100。建议的封闭缓冲液是含有BSA和二抗宿主血清的PBS。建议的稀释缓冲液是含有1 mg/mL BSA的PBS。
ELISA：在直接测定中为1:1,000-1:2,000。
最佳工作稀释度必须由最终用户进行确定。
免疫细胞化学：1:500-1:1000
免疫组织化学：在多聚甲醛固定的组织上以1:500-1:1,000的比例孵育过夜，使用镍增强的DAB进行检测。建议的透化方法是0.5% Triton X-100。建议的封闭缓冲液是含有BSA和二抗宿主血清的PBS。建议的稀释缓冲液是含有1 mg/mL BSA的PBS。
ELISA：在直接测定中为1:1,000-1:2,000。
最佳工作稀释度必须由最终用户进行确定。
Biochem/physiol Actions
与绿色荧光蛋白(GFP)反应。该抗体与各种形式的GFP反应，包括eGFP，包括Clonetech和Invitrogen提供的载体中发现的那些。 与RFP没有交叉反应性。YFP尚未经过检验。 这种抗体对于免疫组织化学效果很好。
General description
来自维多利亚多管发光水母（Aequorea victoria）的绿色荧光蛋白（GFP）可用作荧光指示剂，用于监测各种细胞系统（包括活生物体和固定组织）中的基因表达。与其他生物发光报告基因不同，GFP在没有底物、辅因子或其他内在或外在蛋白质的情况下发出荧光。纯化的GFP是一种27 kDa的单体，由238个氨基酸组成，当被蓝色或紫外线激发时会发出绿色荧光（最大发射波长为509 nm）。
GFP的MW为27 kDa，取决于所检测到的融合蛋白的分子量。
Immunogen
GFP-BSA
Other Notes
浓度：关于批次特定浓度请参见检验报告。
Physical form
形式：纯化
液体形式，溶于含1 mg/mL BSA的PBS中。不含防腐剂。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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