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Merck
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MAB3610

Anti-Bub1 Antibody, clone 14H5

clone 14H5, Chemicon®, from mouse

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
14H5, monoclonal
Application:
immunocytochemistry
immunoprecipitation (IP)
western blot
Species reactivity:
primate, human
Citations:
4
Technique(s):
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
O43683

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产品名称

Anti-Bub1 Antibody, clone 14H5, clone 14H5, Chemicon®, from mouse

Quality Level

100

Gene Information

human ... BUB1(699)

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

14H5, monoclonal

species reactivity

primate, human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_004336.2

UniProt accession no.

O43683

shipped in

wet ice

target post-translational modification

unmodified

Application

Detect Bub1 with Anti-Bub1 Antibody, clone 14H5 (Mouse Monoclonal Antibody), that has been shown to work in IP, WB, ICC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Western blot using an ECL detection system. Reacts with the ~150 kDa Bub1 protein.

Immunocytochemistry: 1 μg/mL on tissue culture cells. Suggested fix is 3.5% PBS-buffered paraformaldehyde for 7 minutes. Permeabilization method is 0.2% Triton-X-100 after fixation. Suggested blocking buffer is 10 mM Tris, pH 7.5 with 150 mM NaCl with 0.1% BSA.

Immunoprecipitation: 1 μg/mL. Preferred cell lysis buffer is RIPA. Final reaction volume is 200 μL with a final protein concentration in the reaction mix of 1 mg/mL. Suggested capture agent is Protein G.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes human Bub1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The mitotic checkpoint prevents the resulting two daughter cells from having unequal numbers of chromosomes. This condition, known as aneuploidy, is present in almost all cancer cells and may be due to malfunctions in the mitotic checkpoint. Bub1 is a mitotic checkpoint kinase and has been implicated in the metaphase checkpoint control in mammalian cells.

Immunogen

Recombinant human Bub1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Mouse IgG1 in 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% sodium azide.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Erica Di Cesare et al.
Oncotarget, 8(12), 19738-19759 (2017-02-06)
Tubulin-targeting molecules are widely used cancer therapeutic agents. They inhibit microtubule-based structures, including the mitotic spindle, ultimately preventing cell division. The final fates of microtubule-inhibited cells are however often heterogeneous and difficult to predict. While recent work has provided insight
Emanuele Roscioli et al.
The Journal of cell biology, 196(4), 435-450 (2012-02-15)
Importin-β is the main vector for interphase nuclear protein import and plays roles after nuclear envelope breakdown. Here we show that importin-β regulates multiple aspects of mitosis via distinct domains that interact with different classes of proteins in human cells.
Marco A Andonegui-Elguera et al.
Cell death discovery, 2, 16079-16079 (2016-11-08)
Spindle poisons activate the spindle assembly checkpoint and prevent mitotic exit until cells die or override the arrest. Several studies have focused on spindle poison-mediated cell death, but less is known about consequences in cells that survive a mitotic arrest.

相关内容

Shaping Epigenetics Discovery - Epigenetics Product Selection Brochure

"Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms instead of by alterations in DNA sequence. These changes can be cell- or tissue-specific, and can be passed on to multiple generations. Epigenetic regulation enriches DNAbased information, allowing a cell to vary its response across diverse biological and environmental contexts. Although epigenetic mechanisms are primarily centered in the nucleus, these mechanisms can be induced by environmental signals such as hormones, nutrients, stress, and cellular damage, pointing to the involvement of cytoplasmic and extracellular factors in epigenetic regulation."

MOUSE ANTI-BUB1
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