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Merck
CN

MAB3610

Anti-Bub1 Antibody, clone 14H5

clone 14H5, Chemicon®, from mouse

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UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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生物来源

mouse

质量水平

100

偶联物

unconjugated

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

14H5, monoclonal

种属反应性

primate, human

制造商/商品名称

Chemicon®

技术

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同位素/亚型

IgG1

NCBI登记号

NM_004336.2

UniProt登记号

O43683

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... BUB1(699)

一般描述

The mitotic checkpoint prevents the resulting two daughter cells from having unequal numbers of chromosomes. This condition, known as aneuploidy, is present in almost all cancer cells and may be due to malfunctions in the mitotic checkpoint. Bub1 is a mitotic checkpoint kinase and has been implicated in the metaphase checkpoint control in mammalian cells.

免疫原

Recombinant human Bub1.

应用

Detect Bub1 with Anti-Bub1 Antibody, clone 14H5 (Mouse Monoclonal Antibody), that has been shown to work in IP, WB, ICC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Western blot using an ECL detection system. Reacts with the ~150 kDa Bub1 protein.

Immunocytochemistry: 1 μg/mL on tissue culture cells. Suggested fix is 3.5% PBS-buffered paraformaldehyde for 7 minutes. Permeabilization method is 0.2% Triton-X-100 after fixation. Suggested blocking buffer is 10 mM Tris, pH 7.5 with 150 mM NaCl with 0.1% BSA.

Immunoprecipitation: 1 μg/mL. Preferred cell lysis buffer is RIPA. Final reaction volume is 200 μL with a final protein concentration in the reaction mix of 1 mg/mL. Suggested capture agent is Protein G.

Optimal working dilutions must be determined by the end user.

生化/生理作用

Recognizes human Bub1.

外形

Format: Purified
Mouse IgG1 in 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% sodium azide.

制备说明

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

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Erica Di Cesare et al.
Oncotarget, 8(12), 19738-19759 (2017-02-06)
Tubulin-targeting molecules are widely used cancer therapeutic agents. They inhibit microtubule-based structures, including the mitotic spindle, ultimately preventing cell division. The final fates of microtubule-inhibited cells are however often heterogeneous and difficult to predict. While recent work has provided insight
Emanuele Roscioli et al.
The Journal of cell biology, 196(4), 435-450 (2012-02-15)
Importin-β is the main vector for interphase nuclear protein import and plays roles after nuclear envelope breakdown. Here we show that importin-β regulates multiple aspects of mitosis via distinct domains that interact with different classes of proteins in human cells.
Marco A Andonegui-Elguera et al.
Cell death discovery, 2, 16079-16079 (2016-11-08)
Spindle poisons activate the spindle assembly checkpoint and prevent mitotic exit until cells die or override the arrest. Several studies have focused on spindle poison-mediated cell death, but less is known about consequences in cells that survive a mitotic arrest.

相关内容

MOUSE ANTI-BUB1
Shaping Epigenetics Discovery - Epigenetics Product Selection Brochure

"Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms instead of by alterations in DNA sequence. These changes can be cell- or tissue-specific, and can be passed on to multiple generations. Epigenetic regulation enriches DNAbased information, allowing a cell to vary its response across diverse biological and environmental contexts. Although epigenetic mechanisms are primarily centered in the nucleus, these mechanisms can be induced by environmental signals such as hormones, nutrients, stress, and cellular damage, pointing to the involvement of cytoplasmic and extracellular factors in epigenetic regulation."

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