MAB3738
抗-PML抗体，克隆36.1-104
ascites fluid, clone 36.1-104, Chemicon®
别名:
Promyelocytic Leukemia Protein, Tripartite Motif Protein 19
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
36.1-104, monoclonal
Application:
ICC, IP, WB
Citations:
34
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
36.1-104, monoclonal
species reactivity
mouse
should not react with
human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2b
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... PML(5371)
General description
106 kDa
PML蛋白是一种含有三结构域基序(TRIM)的核蛋白，除其他外，它可以作为转录因子、核受体的共激活因子（Ruggerio，2000）、细胞凋亡调节剂（Guo，2000）、干扰素诱导的抗病毒反应的介质（Regand和Chelbi-Aliz，2001）以及生长和致癌转化的抑制剂（Mu，1997）发挥作用。PML位于核质和不同的亚核结构中，称为早幼粒细胞白血病体（也称为核结构域10）。将PML定位于早幼粒细胞白血病体需要通过小泛素修饰剂(SUMO)修饰PML蛋白，并且是这些亚核结构正确形成和完整性所必需的。文献中已经描述了至少14种分子量范围为48-97 kDa（预测）的PML剪接变体。各种剪接变体的功能意义尚不十分清楚。在急性早幼粒细胞白血病患者中，PML基因参与至少两种特定的染色体易位，导致与视黄酸受体α（RARα DNA和激素结合域；Pandolfi，2001）嵌合蛋白的表达。在A型和B型APL中表达的PML的所有亚型以及PML-RARalpha嵌合蛋白含有相同的N末端，但在蛋白的C末端部分不同（Jenson，2001）。
Immunogen
His标记的PML融合蛋白，对应于小鼠PML的氨基酸1-581。
Application
抗PML抗体（克隆36.1-104）是一种用于检测PML（也称为早幼粒细胞白血病蛋白）的小鼠单克隆抗体，&已在ICC、IP & WB中得到验证。
研究子类别
转录因子
转录因子
研究类别
表观遗传学&核功能
表观遗传学&核功能
蛋白质印迹（1:500）
免疫沉淀
免疫细胞化学（1:100）
最佳工作稀释度必须由最终用户确定。
免疫沉淀
免疫细胞化学（1:100）
最佳工作稀释度必须由最终用户确定。
Biochem/physiol Actions
识别小鼠PML（早幼粒细胞白血病蛋白）。在来自小鼠胚胎成纤维细胞（MEF1细胞）的蛋白质提取物的蛋白质印迹上，MAB3738识别对应于PML的以约106 kDa迁移的条带。
Physical form
不含防腐剂的腹水。
未纯化
Preparation Note
自收到之日起在-20°C可稳定保存1年。分装保存以避免反复冻融。为了最大程度地回收产品，需在融化后和取下盖子之前将原始样品管进行离心。
Analysis Note
对照
阳性对照：小鼠胚胎成纤维细胞（MEF1细胞）。
阳性对照：小鼠胚胎成纤维细胞（MEF1细胞）。
Other Notes
浓度：关于批次特定浓度请参见检验报告。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Marie-Odile Baudement et al.
Genome research, 28(11), 1733-1746 (2018-10-06)
The mammalian cell nucleus contains numerous discrete suborganelles named nuclear bodies. While recruitment of specific genomic regions into these large ribonucleoprotein (RNP) complexes critically contributes to higher-order functional chromatin organization, such regions remain ill-defined. We have developed the high-salt-recovered sequences-sequencing
Arkadiy K Golov et al.
PloS one, 10(10), e0139855-e0139855 (2015-10-06)
The extremely high concentration of macromolecules in a eukaryotic cell nucleus indicates that the nucleoplasm is a crowded macromolecular solution in which large objects tend to gather together due to crowding forces. It has been shown experimentally that crowding forces
Tracy Dagher et al.
The Journal of experimental medicine, 218(2) (2020-10-20)
Interferon α (IFNα) is used to treat JAK2V617F-driven myeloproliferative neoplasms (MPNs) but rarely clears the disease. We investigated the IFNα mechanism of action focusing on PML, an interferon target and key senescence gene whose targeting by arsenic trioxide (ATO) drives
Ming Chen et al.
Nature genetics, 50(2), 206-218 (2018-01-18)
Lipids, either endogenously synthesized or exogenous, have been linked to human cancer. Here we found that PML is frequently co-deleted with PTEN in metastatic human prostate cancer (CaP). We demonstrated that conditional inactivation of Pml in the mouse prostate morphs
Michiko Niwa-Kawakita et al.
The Journal of experimental medicine, 214(11), 3197-3206 (2017-09-22)
Promyelocytic leukemia (PML) nuclear bodies (NBs) recruit partner proteins, including p53 and its regulators, thereby controlling their abundance or function. Investigating arsenic sensitivity of acute promyelocytic leukemia, we proposed that PML oxidation promotes NB biogenesis. However, physiological links between PML
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