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Merck
CN

MAB3776

Anti-MDM2 Antibody, clone SMP14

clone SMP14, Chemicon®, from mouse

别名:

Murine Double Minute 2

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
SMP14, monoclonal
Conjugate:
unconjugated
Application:
IHC (p), IP
Citations:
4

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clone

SMP14, monoclonal

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

species reactivity

rat, human, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable

isotype

IgG1

NCBI accession no.

NM_002392.2, NM_006878.2, NM_006879.2, NM_006881.2, NM_006882.2

UniProt accession no.

Q00987

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... MDM2(4193)
mouse ... Mdm2(17246)
rat ... Mdm2(314856)

General description

MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated (Zhang & Prives, 2001). SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.

Immunogen

Synthetic peptide corresponding to residues 154-167 (CSRPSTSSRRRAISE) of human MDM2 cross-linked to KLH using glutaldehyde.

Application

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

RNA Binding Protein (RBP)
Use Anti-MDM2 Antibody, clone SMP14 (Mouse Monoclonal Antibody) validated in IP, IHC, IHC(P) to detect MDM2 also known as Murine Double Minute 2.
Western blot

Immunohistochemistry (Frozen and Paraffin-embedded sections)

Immunoprecipitation

ELISA

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Recognizes human MDM2.

Physical form

Format: Purified
Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide

Analysis Note

Control
POSITIVE CONTROL: Breast carcinoma

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Richard O Adeyemi et al.
Journal of virology, 86(15), 8328-8332 (2012-05-25)
The DNA damage response to infection with minute virus of mice (MVM) leads to activated p53; however, p21 levels are reduced via a proteasome-mediated mechanism. This loss was sustained, as virus replicated in infected cells held at the G(2)/M border.
Gengze Wu et al.
Circulation, 130(17), 1452-1465 (2014-08-27)
Long noncoding RNAs (lncRNAs) have recently been implicated in many biological processes and diseases. Atherosclerosis is a major risk factor for cardiovascular disease. However, the functional role of lncRNAs in atherosclerosis is largely unknown. We identified lincRNA-p21 as a key

相关内容

RNA-Binding Protein Immunoprecipitation

All eukaryotic organisms require tight regulation of gene expression for complex processes such as development, differentiation, cell specification, and responses to environmental stimuli. Many genes are regulated post-transcriptionally, in addition to transcriptional mechanisms of gene regulation. RNA-binding proteins (RBPs) are essential for post-transcriptional gene regulation, linking transcription and translation in many processes including transcription, splicing, export, rate of translation and turnover. In all of these events, RBPs coordinate regulation of the amount of protein produced from mRNA transcripts.

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