MAB3802
抗-53BP1抗体，克隆BP13
clone BP13, Chemicon®, from mouse
别名:
p53 Binding Protein 1
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
BP13, monoclonal
Application:
IF, WB
Citations:
76
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
BP13, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TP53BP1(7158)
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General description
P53结合蛋白1（53BP1）在肿瘤抑制中起关键作用，并且是ATM激酶的假定底物。DNA损伤后，它被磷酸化并重新定位到损伤的推测位点，特别是双链断裂。这也暗示了在DNA修复和维持基因组稳定性中的作用。它是序列特异性转录因子，在哺乳动物细胞对遗传毒性应激的反应中起着核心作用。同样，53BP1定位于有丝分裂细胞中的动植物，表明在有丝分裂检查点信号传导中具有潜在功能。
Immunogen
重组人53BP1蛋白。
Application
用IF&WB中验证的抗53BP1抗体（克隆BP13（小鼠单克隆抗体））检测53BP1（也称为p53结合蛋白1）。
研究子类别
细胞周期、DNA复制&修复
细胞周期、DNA复制&修复
研究类别
表观遗传学&核功能
表观遗传学&核功能
蛋白质印迹法：识别>250 kD的蛋白质。建议的稀释缓冲液为TBST。建议的封闭缓冲液为5%脱脂奶粉。
免疫沉淀。免疫印迹的预期大小为>250 kD。建议的裂解缓冲液为NETN。建议的捕获剂是蛋白A+兔抗小鼠接头抗体。
最佳工作稀释度必须由最终用户进行确定。
免疫沉淀。免疫印迹的预期大小为>250 kD。建议的裂解缓冲液为NETN。建议的捕获剂是蛋白A+兔抗小鼠接头抗体。
最佳工作稀释度必须由最终用户进行确定。
Biochem/physiol Actions
53BP1
Physical form
形式：纯化
纯化的免疫球蛋白。PBS中的液体，含有叠氮化钠。
Preparation Note
自接收之日起，以未稀释的等分试样在2-8°C下保存长达12个月。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Ankana Tiwari et al.
Nature communications, 9(1), 677-677 (2018-02-16)
Chromosome missegregation acts as one of the driving forces for chromosome instability and cancer development. Here, we find that in human cancer cells, HeLa and U2OS, depletion of 53BP1 (p53-binding protein 1) exacerbates chromosome non-disjunction resulting from a new type
Grasiella Angelina Andriani et al.
Scientific reports, 6, 35218-35218 (2016-10-13)
Age-related accumulation of ploidy changes is associated with decreased expression of genes controlling chromosome segregation and cohesin functions. To determine the consequences of whole chromosome instability (W-CIN) we down-regulated the spindle assembly checkpoint component BUB1 and the mitotic cohesin SMC1A
Shuang Liu et al.
FEBS open bio, 9(2), 304-314 (2019-02-15)
RNA splicing has emerged as a critical player in the DNA damage response (DDR). However, the underlying mechanism(s) by which pre-mRNA splicing is coordinately regulated by genotoxic stress has remained largely unclear. Here, we show that a DDR factor, DNA-dependent
Chao Wang et al.
Oncogene, 38(14), 2451-2463 (2018-12-12)
Ataxia telangiectasia mutated and RAD3 related (ATR) protein kinase plays critical roles in ensuring DNA replication, DNA repair, and cell cycle control in response to replication stress, making ATR inhibition a promising therapeutic strategy for cancer treatment. To identify genes
Man Luo et al.
Aging, 10(4), 549-560 (2018-04-18)
The inflammatory cascade can be initiated with the recognition of damaged DNA. Macrophages play an essential role in particulate matter (PM)-induced airway inflammation. In this study, we aim to explore the PM induced DNA damage response of macrophages and its
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