产品名称
Anti-53BP1 Antibody, clone BP18, ascites fluid, clone BP18, Chemicon®
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
BP18, monoclonal
species reactivity
mouse, human
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable
western blot: suitable
isotype
IgM
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TP53BP1(7158)
Application
Anti-53BP1 Antibody, clone BP18 is a Mouse Monoclonal Antibody for detection of 53BP1 also known as p53 Binding Protein 1 & has been validated in IF & WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Western blot. Recognizes a protein of >250 kD. Suggested dilution buffer is TBST. Suggested blocking buffer is 5% non-fat dry milk.
Immunocytochemistry
Immunoprecipitation. Expected size in immunoblot is >250 kD. Suggested lysis buffer is NETN. Suggested capture agent is Protein A + rabbit anti-mouse linker antibody.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry
Immunoprecipitation. Expected size in immunoblot is >250 kD. Suggested lysis buffer is NETN. Suggested capture agent is Protein A + rabbit anti-mouse linker antibody.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
53BP1
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Recombinant human 53BP1 proteins.
Physical form
Ascites. Liquid.
Preparation Note
Maintain at -20°C in undiluted aliquots up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Dejie Wang et al.
Science advances, 7(25) (2021-06-20)
53BP1 activates nonhomologous end joining (NHEJ) and inhibits homologous recombination (HR) repair of DNA double-strand breaks (DSBs). Dissociation of 53BP1 from DSBs and consequent activation of HR, a less error-prone pathway than NHEJ, helps maintain genome integrity during DNA replication;
Claudia E Rübe et al.
NPJ aging and mechanisms of disease, 7(1), 7-7 (2021-04-03)
Cellular senescence is an irreversible growth arrest that occurs as a result of damaging stimuli, including DNA damage and/or telomere shortening. Here, we investigate histone variant H2A.J as a new biomarker to detect senescent cells during human skin aging. Skin
Elisa Varela et al.
Nature communications, 7, 11739-11739 (2016-06-03)
Although telomere length is genetically determined, mouse embryonic stem (ES) cells with telomeres of twice the normal size have been generated. Here, we use such ES cells with 'hyper-long' telomeres, which also express green fluorescent protein (GFP), to generate chimaeric
Deeksha Munnur et al.
Cell reports, 26(8), 2028-2036 (2019-02-21)
Although poly-ADP-ribosylation (PARylation) of DNA repair factors had been well documented, its role in the repair of DNA double-strand breaks (DSBs) is poorly understood. NR4A nuclear orphan receptors were previously linked to DSB repair; however, their function in the process
Ishita Ghosh et al.
Nucleic acids research, 51(16), 8643-8662 (2023-07-13)
Environmental agents like ionizing radiation (IR) and chemotherapeutic drugs can cause severe damage to the DNA, often in the form of double-strand breaks (DSBs). Remaining unrepaired, DSBs can lead to chromosomal rearrangements, and cell death. One major error-free pathway to
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