生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
5.2F, monoclonal
种属反应性
rat, mouse, chicken, human
制造商/商品名称
Chemicon®
技术
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG2a
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... MYOD1(4654)
一般描述
约45 kDa
免疫原
来自小鼠MyoD1的重组蛋白。
应用
抗MyoD1抗体是一种用于检测MyoD1的高质量小鼠单克隆抗体，&已在ICC、IHC、IP & WB中进行了验证。
蛋白质印迹：1 μg/mL。与~45 kD蛋白反应。
针对冷冻和福尔马林固定、石蜡包埋组织的免疫组织化学：2-4 μg/mL。
免疫细胞化学
免疫沉淀：2 μg/mg蛋白裂解物
最佳工作稀释度必须由最终用户确定。
针对冷冻和福尔马林固定、石蜡包埋组织的免疫组织化学：2-4 μg/mL。
免疫细胞化学
免疫沉淀：2 μg/mg蛋白裂解物
最佳工作稀释度必须由最终用户确定。
生化/生理作用
识别MyoD1。该表位位于小鼠MyoD1蛋白N末端的3-56位氨基酸。
外形
形式：纯化
分析说明
对照
胎儿肌肉，横纹肌肉瘤
胎儿肌肉，横纹肌肉瘤
其他说明
浓度：关于批次特定浓度请参见检验报告。
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
A recurrent neomorphic mutation in MYOD1 defines a clinically aggressive subset of embryonal rhabdomyosarcoma associated with PI3K-AKT pathway mutations.
Kohsaka, S; Shukla, N; Ameur, N; Ito, T; Ng, CK; Wang, L; Lim, D; Marchetti, A; Viale et al.
Nature Genetics null
Francesca Megiorni et al.
Journal of hematology & oncology, 10(1), 161-161 (2017-10-08)
EPH (erythropoietin-producing hepatocellular) receptors are clinically relevant targets in several malignancies. This report describes the effects of GLPG1790, a new potent pan-EPH inhibitor, in human embryonal rhabdomyosarcoma (ERMS) cell lines. EPH-A2 and Ephrin-A1 mRNA expression was quantified by real-time PCR
Mark A Gillespie et al.
The Journal of cell biology, 187(7), 991-1005 (2009-12-23)
The mitogen-activated protein kinase p38-gamma is highly expressed in skeletal muscle and is associated with the dystrophin glycoprotein complex; however, its function remains unclear. After induced damage, muscle in mice lacking p38-gamma generated significantly fewer myofibers than wild-type muscle. Notably
L V Renna et al.
European journal of histochemistry : EJH, 58(4), 2444-2444 (2015-01-13)
Myotonic dystrophy type 1 (DM1) and type 2 (DM2) are multisystemic disorders linked to two different genetic loci and characterized by several features including myotonia, muscle weakness and atrophy, cardiac dysfunctions, cataracts and insulin-resistance. In both forms, expanded nucleotide sequences
Junmin Lee et al.
Biomaterials, 69, 174-183 (2015-08-19)
Cells sense and transduce the chemical and mechanical properties of their microenvironment through cell surface integrin receptors. Traction stress exerted by cells on the extracellular matrix mediates focal adhesion stabilization and regulation of the cytoskeleton for directing biological activity. Understanding
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