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Merck
CN

MAB3878

抗-MyoD1抗体

clone 5.2F, Chemicon®, from mouse

别名:

Anti-MYF3, Anti-MYOD, Anti-MYODRIF, Anti-PUM, Anti-bHLHc1

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
5.2F, monoclonal
Application:
immunocytochemistry
immunohistochemistry
immunoprecipitation (IP)
western blot
Species reactivity:
rat, mouse, chicken, human
Citations:
8
Technique(s):
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
P15172

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产品名称

抗-MyoD1抗体, clone 5.2F, Chemicon®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5.2F, monoclonal

species reactivity

rat, mouse, chicken, human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

NM_002478.4

UniProt accession no.

P15172

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... MYOD1(4654)

相关类别

Analysis Note

对照
胎儿肌肉，横纹肌肉瘤

Application

抗MyoD1抗体是一种用于检测MyoD1的高质量小鼠单克隆抗体，&已在ICC、IHC、IP & WB中进行了验证。
蛋白质印迹：1 μg/mL。与~45 kD蛋白反应。

针对冷冻和福尔马林固定、石蜡包埋组织的免疫组织化学：2-4 μg/mL。

免疫细胞化学

免疫沉淀：2 μg/mg蛋白裂解物

最佳工作稀释度必须由最终用户确定。

Biochem/physiol Actions

识别MyoD1。该表位位于小鼠MyoD1蛋白N末端的3-56位氨基酸。

General description

约45 kDa

Immunogen

来自小鼠MyoD1的重组蛋白。

Other Notes

浓度：关于批次特定浓度请参见检验报告。

Physical form

形式：纯化

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Mark A Gillespie et al.
The Journal of cell biology, 187(7), 991-1005 (2009-12-23)
The mitogen-activated protein kinase p38-gamma is highly expressed in skeletal muscle and is associated with the dystrophin glycoprotein complex; however, its function remains unclear. After induced damage, muscle in mice lacking p38-gamma generated significantly fewer myofibers than wild-type muscle. Notably
A recurrent neomorphic mutation in MYOD1 defines a clinically aggressive subset of embryonal rhabdomyosarcoma associated with PI3K-AKT pathway mutations.
Kohsaka, S; Shukla, N; Ameur, N; Ito, T; Ng, CK; Wang, L; Lim, D; Marchetti, A; Viale et al.
Nature Genetics null
In situ mechanotransduction via vinculin regulates stem cell differentiation.
Holle, AW; Tang, X; Vijayraghavan, D; Vincent, LG; Fuhrmann, A; Choi, YS; del Alamo, JC; Engler, AJ
Stem Cells null
Francesca Megiorni et al.
Journal of hematology & oncology, 10(1), 161-161 (2017-10-08)
EPH (erythropoietin-producing hepatocellular) receptors are clinically relevant targets in several malignancies. This report describes the effects of GLPG1790, a new potent pan-EPH inhibitor, in human embryonal rhabdomyosarcoma (ERMS) cell lines. EPH-A2 and Ephrin-A1 mRNA expression was quantified by real-time PCR
L V Renna et al.
European journal of histochemistry : EJH, 58(4), 2444-2444 (2015-01-13)
Myotonic dystrophy type 1 (DM1) and type 2 (DM2) are multisystemic disorders linked to two different genetic loci and characterized by several features including myotonia, muscle weakness and atrophy, cardiac dysfunctions, cataracts and insulin-resistance. In both forms, expanded nucleotide sequences
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