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Merck
CN

MAB4065

Anti-H-CAM Antibody, Pan, clone SFF-2

clone SFF-2, Chemicon®, from mouse

别名:

CD44, CD44std, CD44H, HCAM

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
SFF-2, monoclonal
Application:
FACS, ICC, IHC (p)
Species reactivity:
human
Citations:
1
Technique(s):
flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
Uniprot accession no.:
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biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

SFF-2, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

isotype

IgG1κ

suitability

not suitable for Western blot

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... CD44(960)

Analysis Note

Control
POSITIVE CONTROL: Lymph node (lymphocytes), skin (keratinocytes), or tonsil.

Application

Immunohistochemistry: 1 µg/mL; acetone fixed cryostat sections; 2 µg/mL following heat-induced epitope retrieval in 0.01 M citrate buffer, pH 6.0 (Banfalvi, 1999).
Immunofluorescence
Flow Cytometry: 1:20 as determined on U937 cells
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
This Anti-H-CAM Antibody, Pan, clone SFF-2 is validated for use in FC, IC, IH, IH(P) for the detection of H-CAM.

Biochem/physiol Actions

Specific for human standard CD44. Does not distinguish between splice variants (exons v3v10).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: Pan

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1123

Physical form

Format: Purified
Liquid in PBS.

Preparation Note

Prior to opening maintain at -20°C until expiration date. After opening maintain frozen at -20°C in undiluted aliquots until expiration date. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Young Sook Lee et al.
Journal of controlled release : official journal of the Controlled Release Society, 205, 128-133 (2015-01-13)
Multipotent mesenchymal stem cells (MSCs) promise a therapeutic alternative for many debilitating and incurable diseases. However, one of the major limitations for the therapeutic application of human MSC (hMSC) is the lengthy ex vivo expansion time for preparing a sufficient

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