Anti-MRP1 Antibody, clone QCRL-1

Reacts with a defined linear epitope (aa 918-924) of MRP1, a 190 kDa transmembrane

phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines

and tumors (Cole et al., 1992; Hipfner et al., 1994, 1996, 1999; Wright et al, 1998). Does not cross react with the mouse mrp1, or human MDR1, MDR3,

MRP2, MRP3, MRP4, MRP5 or MRP6 gene products.

Non-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1 (Cole et al., 1992; Hipfner et al., 1994, 1999).

Anti-MRP1 Antibody, clone QCRL-1 detects level of MRP1 & has been published & validated for use in FC, WB, IC, IH, IH(P).

Western Blot: 0.2 μg/mL, with HRP-conjugated anti mouse IgG (Hipfner et al., 1994, 1996).

Immunocytochemistry: 1:20 - 1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations (Hipfner et al., 1999).

Immunohistochemistry on formalin-fixed paraffini-embedded sections following antigen retrieval by microwave heating in citrate (Wright, et al., 1998).

Flow cytometry: 1:20 dilution on cells fixed in 0.5% paraformaldehyde in PBS for 30 minutes, followed by anti-mouse FITC (block and wash with solution containing 0.1% Tween) (Hipfner, et al., 1994).

Immunoprecipitation (Hipfner, et al., 1994; 1996).

Optimal working dilutions must be determined by the end user.

Format: Purified

Purified immunoglobulin. Liquid in PBS with 0.1% sodium azide.

Store at 2-8ºC for up to 3 months. For longer term storage, aliquot and freeze at -20ºC.

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