生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
QCRL-1, monoclonal
种属反应性
human
制造商/商品名称
Chemicon®
技术
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型
IgG1
UniProt登记号
运输
wet ice
基因信息
human ... ABCC1(4363)
免疫原
Non-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1 (Cole et al., 1992; Hipfner et al., 1994, 1999).
应用
Anti-MRP1 Antibody, clone QCRL-1 detects level of MRP1 & has been published & validated for use in FC, WB, IC, IH, IH(P).
Western Blot: 0.2 μg/mL, with HRP-conjugated anti mouse IgG (Hipfner et al., 1994, 1996).
Immunocytochemistry: 1:20 - 1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations (Hipfner et al., 1999).
Immunohistochemistry on formalin-fixed paraffini-embedded sections following antigen retrieval by microwave heating in citrate (Wright, et al., 1998).
Flow cytometry: 1:20 dilution on cells fixed in 0.5% paraformaldehyde in PBS for 30 minutes, followed by anti-mouse FITC (block and wash with solution containing 0.1% Tween) (Hipfner, et al., 1994).
Immunoprecipitation (Hipfner, et al., 1994; 1996).
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:20 - 1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations (Hipfner et al., 1999).
Immunohistochemistry on formalin-fixed paraffini-embedded sections following antigen retrieval by microwave heating in citrate (Wright, et al., 1998).
Flow cytometry: 1:20 dilution on cells fixed in 0.5% paraformaldehyde in PBS for 30 minutes, followed by anti-mouse FITC (block and wash with solution containing 0.1% Tween) (Hipfner, et al., 1994).
Immunoprecipitation (Hipfner, et al., 1994; 1996).
Optimal working dilutions must be determined by the end user.
生化/生理作用
Reacts with a defined linear epitope (aa 918-924) of MRP1, a 190 kDa transmembrane
phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines
and tumors (Cole et al., 1992; Hipfner et al., 1994, 1996, 1999; Wright et al, 1998). Does not cross react with the mouse mrp1, or human MDR1, MDR3,
MRP2, MRP3, MRP4, MRP5 or MRP6 gene products.
phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines
and tumors (Cole et al., 1992; Hipfner et al., 1994, 1996, 1999; Wright et al, 1998). Does not cross react with the mouse mrp1, or human MDR1, MDR3,
MRP2, MRP3, MRP4, MRP5 or MRP6 gene products.
外形
Format: Purified
Purified immunoglobulin. Liquid in PBS with 0.1% sodium azide.
制备说明
Store at 2-8ºC for up to 3 months. For longer term storage, aliquot and freeze at -20ºC.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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