MAB4100
Anti-MRP1 Antibody, clone QCRL-1
clone QCRL-1, Chemicon®, from mouse
别名:
Multidrug Resistance Protein-1
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
QCRL-1, monoclonal
Species reactivity:
human
Application:
flow cytometry
immunocytochemistry
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
western blot
immunocytochemistry
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
western blot
Technique(s):
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
Citations:
19
Uniprot accession no.:
产品名称
Anti-MRP1 Antibody, clone QCRL-1, clone QCRL-1, Chemicon®, from mouse
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
QCRL-1, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
wet ice
Quality Level
Gene Information
human ... ABCC1(4363)
Application
Anti-MRP1 Antibody, clone QCRL-1 detects level of MRP1 & has been published & validated for use in FC, WB, IC, IH, IH(P).
Western Blot: 0.2 μg/mL, with HRP-conjugated anti mouse IgG (Hipfner et al., 1994, 1996).
Immunocytochemistry: 1:20 - 1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations (Hipfner et al., 1999).
Immunohistochemistry on formalin-fixed paraffini-embedded sections following antigen retrieval by microwave heating in citrate (Wright, et al., 1998).
Flow cytometry: 1:20 dilution on cells fixed in 0.5% paraformaldehyde in PBS for 30 minutes, followed by anti-mouse FITC (block and wash with solution containing 0.1% Tween) (Hipfner, et al., 1994).
Immunoprecipitation (Hipfner, et al., 1994; 1996).
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:20 - 1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations (Hipfner et al., 1999).
Immunohistochemistry on formalin-fixed paraffini-embedded sections following antigen retrieval by microwave heating in citrate (Wright, et al., 1998).
Flow cytometry: 1:20 dilution on cells fixed in 0.5% paraformaldehyde in PBS for 30 minutes, followed by anti-mouse FITC (block and wash with solution containing 0.1% Tween) (Hipfner, et al., 1994).
Immunoprecipitation (Hipfner, et al., 1994; 1996).
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Reacts with a defined linear epitope (aa 918-924) of MRP1, a 190 kDa transmembrane
phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines
and tumors (Cole et al., 1992; Hipfner et al., 1994, 1996, 1999; Wright et al, 1998). Does not cross react with the mouse mrp1, or human MDR1, MDR3,
MRP2, MRP3, MRP4, MRP5 or MRP6 gene products.
phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines
and tumors (Cole et al., 1992; Hipfner et al., 1994, 1996, 1999; Wright et al, 1998). Does not cross react with the mouse mrp1, or human MDR1, MDR3,
MRP2, MRP3, MRP4, MRP5 or MRP6 gene products.
Immunogen
Non-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1 (Cole et al., 1992; Hipfner et al., 1994, 1999).
Physical form
Format: Purified
Purified immunoglobulin. Liquid in PBS with 0.1% sodium azide.
Preparation Note
Store at 2-8ºC for up to 3 months. For longer term storage, aliquot and freeze at -20ºC.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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A rapid and quantitative coat protein complex II vesicle formation assay using luciferase reporters.
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S P Cole et al.
Science (New York, N.Y.), 258(5088), 1650-1654 (1992-12-04)
The doxorubicin-selected lung cancer cell line H69AR is resistant to many chemotherapeutic agents. However, like most tumor samples from individuals with this disease, it does not overexpress P-glycoprotein, a transmembrane transport protein that is dependent on adenosine triphosphate (ATP) and
Cells resistant to HTI-286 do not overexpress P-glycoprotein but have reduced drug accumulation and a point mutation in alpha-tubulin.
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