biological source
mouse
Quality Segment
antibody form
culture supernatant
antibody product type
primary antibodies
clone
P3 II-26, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG2b
suitability
not suitable for immunohistochemistry (Paraffin)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
Gene Information
human ... ABCB4(5244)
Immunogen
Mab was selected after immunization with a fusion protein consisting of Gluthatione-S-Transferase and a fragment of MDR3 P-gp comprising aa 629-692.
Application
Anti-MDR3 Antibody, clone P3 II-26 is an antibody against MDR3 for use in WB, IC, IH.
Western Blotting: MDR3 is a 140 kDa protein.
Immunocytochemistry: 1:20 - 1:50 on acetone-fixed cytospin preparations
Immunohistochemistry: 1:20 on acetone-fixed frozen sections
Not suitable for paraffin-embedded tissues
Optimal working dilutions must be determined by end user.
Immunocytochemistry: 1:20 - 1:50 on acetone-fixed cytospin preparations
Immunohistochemistry: 1:20 on acetone-fixed frozen sections
Not suitable for paraffin-embedded tissues
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
This antibody reacts with an internal epitope of MDR3 P-gp. It does not cross-react with the human MDR1 P-gp. It has been reported that this clone also cross reacts with mouse MDR2 Pgp {Kok et al 2003 {http://www.biochemj.org/bj/369/0539/3690539.pdf}.
Preparation Note
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2