MAB4401
Anti-Oct-4 Antibody, clone 10H11.2
clone 10H11.2, Chemicon®, from mouse
别名:
Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3
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所有图片(7)
About This Item
UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41
生物来源
mouse
抗体形式
purified antibody
克隆
10H11.2, monoclonal
种属反应性
human
制造商/商品名称
Chemicon®
技术
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
输入
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
同位素/亚型
IgG1
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... POU5F1(5460)
一般描述
Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
Purified monoclonal antibody for the detection of Human Oct-4.
特异性
Reacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells).
The clone reacts strongly with specific nuclear binding to human ES cells and cross-reacts weakly with mouse ES cells.
应用
Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2 validated for use in WB, FC, IC, ELISA.
Flow Cytometry: A previous lot of this antibody was used in FC.
ELISA: A previous lot of this antibody was used in ELISA.
Immunocytochemistry: A previous lot of this antibody was used in IC. H9 cells in culture on a mouse embronic fibroblast feeder layer were labeled by a standard indirect IF protocol. Oct-4 labelling in green, DAPI in blue, & SSEA-3 (MAB4303 in red).
Optimal working dilutions must be determined by the end user.
ELISA: A previous lot of this antibody was used in ELISA.
Immunocytochemistry: A previous lot of this antibody was used in IC. H9 cells in culture on a mouse embronic fibroblast feeder layer were labeled by a standard indirect IF protocol. Oct-4 labelling in green, DAPI in blue, & SSEA-3 (MAB4303 in red).
Optimal working dilutions must be determined by the end user.
质量
Evaluated by western blot on mouse P1 brain lysates.
Western Blotting: 1:500 dilution of this antibody detected Oct-4 on 10 µg of mouse P1 brain lysates
Western Blotting: 1:500 dilution of this antibody detected Oct-4 on 10 µg of mouse P1 brain lysates
目标描述
44 kDa
外形
Format: Purified
分析说明
Control
Human embryonic stem cell lysate.
Human embryonic stem cell lysate.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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