产品名称
Anti-Bcl-X Antibody, clone 2H12, clone 2H12, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2H12, monoclonal
species reactivity
rat, pig, mouse, human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... BCL2L1(598)
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
BCL2 & Inhibition
BCL2 & Inhibition
This Anti-Bcl-X Antibody, clone 2H12 is validated for use in FC, IF, IP, WB, IH for the detection of Bcl-X.
Wstern Blot: 1μg/mL (Hsu, 1997a; Hsu, 1997b)
Flow Cytometry
Immunofluorescence Immunoprecipitation: native protein (2ug/mg protein lysate).
Immunohistochemistry (frozen and paraffin): 2-4ug/mL for 30 minutes at room temperature.
Optimal working dilutions must be determined by end user.
Flow Cytometry
Immunofluorescence Immunoprecipitation: native protein (2ug/mg protein lysate).
Immunohistochemistry (frozen and paraffin): 2-4ug/mL for 30 minutes at room temperature.
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
MAB4625 recognizes a protein of 27 kDa, identified as the Bcl-X protein. Reacts with both Bcl-XS and Bcl-XL.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Bcl-X has two isoforms, Bcl-XL (long), a 241-aa protein, and Bcl-XS (short), a 178-aa protein lacking a 63 aa domain that is well conserved among members of the Bcl-2 family.
Immunogen
Synthetic peptide, aa 3-14 of human Bcl-X protein. This amino acid sequence is shared by human and murine Bcl-X protein.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Protein A purified immunoglobulin in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Preparation Note
Maintain at 2-8°C for up to 12 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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