产品名称
抗Nestin抗体,克隆10C2, clone 10C2, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
clone
10C2, monoclonal
species reactivity
human
should not react with
rat, mouse
packaging
antibody small pack of 25 μg
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
input
sample type epithelial cells
sample type neural stem cell(s)
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
相关类别
Analysis Note
阳性对照:WI-38(ATCC#CCL75)或U251细胞系
Application
WI-38(ATCC#CCL75)可用作阳性对照。U251细胞系也可作为阳性对照。
免疫细胞化学:以1:200的稀释度用于固定在4%多聚甲醛中并用0.02%Triton渗透的细胞上
免疫组织化学:低聚甲醛是首选的固定剂:以1:200的稀释度用于福尔马林固定、石蜡包埋的组织切片。仅封闭过程中使用0.02%triton;稀释缓冲液含0.1%BSA;室温孵育1-2小时。建议将人胎脑作为阳性对照。(CA Messam et al, Develop.Brain Res, 134:87-92, 2002; Gu, et al., Brain Res. 943:174-180, 2002).细胞质中的丝状染色
最佳工作稀释液必须由最终用户进行确定。
神经干细胞
干细胞研究
Biochem/physiol Actions
Disclaimer
General description
Immunogen
Other Notes
Physical form
Preparation Note
在运输过程中,少量产品有时会夹在产品瓶的密封中。对于体积为200μL或更小的产品,我们建议在小瓶的硬质表面上轻轻敲打,或在台式离心机中短暂离心以收集容器′盖中的所有液体。
Legal Information
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
法规信息
商品
Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.
ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes.
Fibroblast growth factors (FGFs) regulate developmental pathways and mesoderm/ectoderm patterning in early embryonic development.
实验方案
A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
相关内容
As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.
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