生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
克隆
10C2, monoclonal
种属反应性
human
请勿与下列物质发生反应
rat, mouse
包装
antibody small pack of 25 μg
制造商/商品名称
Chemicon®
技术
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
输入
sample type epithelial cells
sample type neural stem cell(s)
同位素/亚型
IgG1
NCBI登记号
UniProt登记号
运输
ambient
储存温度
2-8°C
靶向翻译后修饰
unmodified
一般描述
免疫原
应用
WI-38(ATCC#CCL75)可用作阳性对照。U251细胞系也可作为阳性对照。
免疫细胞化学:以1:200的稀释度用于固定在4%多聚甲醛中并用0.02%Triton渗透的细胞上
免疫组织化学:低聚甲醛是首选的固定剂:以1:200的稀释度用于福尔马林固定、石蜡包埋的组织切片。仅封闭过程中使用0.02%triton;稀释缓冲液含0.1%BSA;室温孵育1-2小时。建议将人胎脑作为阳性对照。(CA Messam et al, Develop.Brain Res, 134:87-92, 2002; Gu, et al., Brain Res. 943:174-180, 2002).细胞质中的丝状染色
最佳工作稀释液必须由最终用户进行确定。
神经干细胞
干细胞研究
生化/生理作用
外形
制备说明
在运输过程中,少量产品有时会夹在产品瓶的密封中。对于体积为200μL或更小的产品,我们建议在小瓶的硬质表面上轻轻敲打,或在台式离心机中短暂离心以收集容器′盖中的所有液体。
分析说明
阳性对照:WI-38(ATCC#CCL75)或U251细胞系
其他说明
法律信息
免责声明
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
法规信息
商品
Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.
ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes.
Fibroblast growth factors (FGFs) regulate developmental pathways and mesoderm/ectoderm patterning in early embryonic development.
实验方案
A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
相关内容
As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.
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