产品名称
Anti-Choline Acetyltransferase Antibody, clone 28C4, clone 28C4, Chemicon®, from mouse
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
28C4, monoclonal
species reactivity
human, rat, guinea pig, monkey
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CHAT(1103)
rat ... Chat(290567)
rhesus monkey ... Chat(709977)
Application
Anti-Choline Acetyltransferase Antibody, clone 28C4 detects level of Choline Acetyltransferase & has been published & validated for use in ELISA, IH & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neuronal & Glial Markers
Neurotransmitters & Receptors
Neuronal & Glial Markers
Western blotting: 1:1,000-1:10,000 using a high sensitivity ECL kit. Recognizes a protein of ~67 kDa. May also recognize a protein of 35 kDa which is thought to be a degradation product. Suggested dilution buffer is TBS containing 5% skim milk powder and 0.05% Tween 20.
Immunohistochemistry on free-floating frozen sections of Rhesus monkey brain fixed with 4% paraformaldehyde and coronal vibratome sections of rat brain fixed with 4% paraformaldehyde and 0.2% glutaraldehyde.
ELISA (two side)
Optimal working dilutions must be determined by end user.
Immunohistochemistry on free-floating frozen sections of Rhesus monkey brain fixed with 4% paraformaldehyde and coronal vibratome sections of rat brain fixed with 4% paraformaldehyde and 0.2% glutaraldehyde.
ELISA (two side)
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Reacts with Choline Acetyltransferase (ChAT).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Purified human placenta ChAT.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Purified immunoglobulin. Liquid.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
未找到合适的产品?
试试我们的产品选型工具.
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ARG1-expressing microglia show a distinct molecular signature and modulate postnatal development and function of the mouse brain.
Stratoulias, et al.
Nature Neuroscience, 26, 1008-1020 (2023)
Zhaoqin Huang et al.
Molecular medicine reports, 11(5), 3285-3290 (2015-01-17)
The present study was conducted to investigate the 11C‑Choline metabolic mechanism and examine the association between 11C‑Choline metabolism and uptake in different pathological types of lung cancer. A total of 18 tumor specimens and corresponding normal lung tissues were collected
Katarina Zvarova et al.
The Journal of comparative neurology, 489(4), 501-517 (2005-07-19)
We examined the distribution and fate of cocaine- and amphetamine-regulated transcript peptide (CARTp)(55-102)-immunoreactive (IR) structures in the neonatal and adult rat urinary bladder. Double-labeling studies examining CARTp with tyrosine hydroxylase (TH), neuronal nitric oxide synthase (nNOS), or choline acetyltransferase (ChAT)
Hanqing Tang et al.
International heart journal, 62(3), 647-657 (2021-05-18)
Bioinformatics analysis showed that miR-448-5p expression in the myocardial tissue of rats with myocardial infarction significantly increased, suggesting that it may participate in myocardial cell apoptosis in myocardial infarction. This study aimed to explore the protective effects of miR-448-5p on
Bing Wang et al.
Disease markers, 2022, 2442094-2442094 (2022-05-27)
Both PCAT19 and miR-25-3p have been reported in lung cancer studies, but whether there is a correlation between the two and whether they jointly regulate the progress of lung cancer have not been reported yet. Therefore, this study carried out
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持