MAB5356
抗-视紫红质抗体，CT，最后9个氨基酸，克隆Rho 1D4
clone Rho 1D4, Chemicon®, from mouse
别名:
Anti-CSNBAD1, Anti-OPN2, Anti-RP4
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选择尺寸
关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
Rho 1D4, monoclonal
Application:
immunocytochemistry
immunohistochemistry
immunoprecipitation (IP)
western blot
immunohistochemistry
immunoprecipitation (IP)
western blot
Species reactivity:
vertebrates
Citations:
106
Technique(s):
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
产品名称
抗-视紫红质抗体，CT，最后9个氨基酸，克隆Rho 1D4, clone Rho 1D4, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
Rho 1D4, monoclonal
species reactivity
vertebrates
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... RHO(6010)
General description
48 kDa
Immunogen
牛视紫红质
表位：C末端，最后9个氨基酸
Analysis Note
对照
眼睛
眼睛
Application
抗视紫红质抗体，C末端，最后9个氨基酸，克隆Rho 1D4检测视紫红质水平，&已发布&验证可用于IC、IH、IP & WB。
研究子类别
感官 & PNS
感官 & PNS
研究类别
神经科学
神经科学
蛋白质印迹：1:100-1:500，使用ECL。建议的稀释缓冲液是PBS，封闭缓冲液是含有5%牛奶的PBS。识别36 kDa单体、二聚体和三聚体（取决于样品制备）。煮沸诱导视紫红质的聚集。
用rho 1D4抗体标记视紫红质（约占视网膜感光细胞外节膜盘蛋白的85%）的分离的牛视网膜感光细胞外节膜盘的蛋白质印迹。 SDS凝胶上的视网膜感光细胞外节膜盘数量: (a)2.5 mg；(b)0.63 mg；(c)0.16 mg；(d)0.04 mg。在较高的蛋白质含量下，可以观察到视紫红质的二聚体、三聚体和四聚体以及单体。
免疫组织化学/细胞化学：1:100-1:500在多聚甲醛和戊二醛固定的冰冻组织切片上。优选的固定是在2-8°C下用多聚甲醛固定4小时。建议的透化方法是0.2% Triton X-100。
免疫沉淀
最佳工作稀释度必须由最终用户确定。
用rho 1D4抗体标记视紫红质（约占视网膜感光细胞外节膜盘蛋白的85%）的分离的牛视网膜感光细胞外节膜盘的蛋白质印迹。 SDS凝胶上的视网膜感光细胞外节膜盘数量: (a)2.5 mg；(b)0.63 mg；(c)0.16 mg；(d)0.04 mg。在较高的蛋白质含量下，可以观察到视紫红质的二聚体、三聚体和四聚体以及单体。
免疫组织化学/细胞化学：1:100-1:500在多聚甲醛和戊二醛固定的冰冻组织切片上。优选的固定是在2-8°C下用多聚甲醛固定4小时。建议的透化方法是0.2% Triton X-100。
免疫沉淀
最佳工作稀释度必须由最终用户确定。
Biochem/physiol Actions
识别视紫红质。与天然和重组蛋白反应。对其他蛋白质没有已知的反应性。
Disclaimer
除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。
Other Notes
浓度：关于批次特定浓度请参见检验报告。
Physical form
形式：纯化
纯化蛋白A
蛋白A纯化的小鼠免疫球蛋白，溶于20 mM磷酸钠、250 mM NaCl（pH.7.6）中，含0.1%叠氮化钠作为防腐剂。
Preparation Note
自发运之日起，在2–8°C条件下可稳定保存1年。分装保存以避免反复冻融。为了最大程度地回收产品，需在融化后和取下盖子之前将原始样品管进行离心。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
M Keith Wyatt et al.
PloS one, 8(6), e68088-e68088 (2013-07-11)
Age-related macular degeneration (AMD) is a major cause of vision loss. It is associated with development of characteristic plaque-like deposits (soft drusen) in Bruch's membrane basal to the retinal pigment epithelium (RPE). A sequence variant (Y402H) in short consensus repeat
Bernice H Wong et al.
The Journal of biological chemistry, 291(20), 10501-10514 (2016-03-25)
Eye photoreceptor membrane discs in outer rod segments are highly enriched in the visual pigment rhodopsin and the ω-3 fatty acid docosahexaenoic acid (DHA). The eye acquires DHA from blood, but transporters for DHA uptake across the blood-retinal barrier or
Tomomi Sakai et al.
Journal of autoimmunity, 81, 13-23 (2017-03-23)
TREX1/DNASE III, the most abundant 3'-5' DNA exonuclease in mammalian cells, is tail-anchored on the endoplasmic reticulum (ER). Mutations at the N-terminus affecting TREX1 DNase activity are associated with autoimmune and inflammatory conditions such as Aicardi-Goutières syndrome (AGS). Mutations in
Laurie L Molday et al.
Methods in molecular biology (Clifton, N.J.), 1177, 1-15 (2014-06-20)
Incorporation of short epitope tags into proteins for recognition by commercially available monoclonal or polyclonal antibodies has greatly facilitated the detection, characterization, localization, and purification of heterologously expressed proteins for structure-function studies. A number of tags have been developed, but
Genetic dissection of TAM receptor-ligand interaction in retinal pigment epithelial cell phagocytosis.
Burstyn-Cohen, T; Lew, ED; Traves, PG; Burrola, PG; Hash, JC; Lemke, G
Neuron null
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