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Merck
CN

MAB5442

Anti-Collapsin Response Mediated Protein 5 Antibody, clone CR-1

culture supernatant, clone CR-1, Chemicon®

别名:

CRMP5, DRP-5, ULIP-6, CRAM

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
CR-1, monoclonal
Application:
IHC, WB
Citations:
1
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biological source

rat

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

CR-1, monoclonal

species reactivity

rat, human, bovine

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable, western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... DPYSL5(56896)

Immunogen

CRMP-5 purified from rat and human brain.

Application

Anti-Collapsin Response Mediated Protein 5 Antibody, clone CR-1 detects level of Collapsin Response Mediated Protein 5 & has been published & validated for use in WB, IH.
Research Category
Neuroscience
Research Sub Category
Growth Cones & Axon Guidance
Western blot: 1:500-1:2,000 on human and rat brain extracts. 1:500-1:2,000 on human small-cell lung carcinoma. 1:1,500-1:6,000 on bovine retina and optic nerve extracts.

Immunohistochemistry: 1:50-1:200 on bovine retina frozen sections fixed with 10% formalin. No reactivity on mouse cerebellum.

Protein isolation: Bound to Bio-Rad′s Affi-gel (cat# 153-6046) to pull out native CRMP-5 from aqueous extracts of brain.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Collapsin response mediated protein-5 (CRMP-5). The antibody binds to a region of the human protein from between ~369 to 564 amino acids.

Physical form

Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Uses of the CR-1 and CR-3 antibodies are covered under pending patent application(s) assigned to the Mayo Foundation for Medical Education and Research (MFMER) and are proprietary to MFMER. Academic not-for-profit research institutions are granted an automatic license with the purchase of this product to use the antibody only for internal, non-sponsored, non-commercial, academic research purposes which license specifically excludes the right to sell, or otherwise transfer the antibody or its derivatives to third parties. In accepting this license, all users acknowledge that the CR-1 and CR-3 antibodies are experimental in nature. MFMER makes no warranties, express or implied of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the CR-1 and CR-3 antibodies, patents or product. All others are invited to request a license from MFMER prior to purchasing this antibody or using it for any purpose. For license information, please contact Mayo Medical Ventures, Office of Technology Commercialization at (507) 284-8878.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Gladiola Goranci-Buzhala et al.
Cell reports, 31(10), 107738-107738 (2020-06-11)
Glioblastoma (GBM) possesses glioma stem cells (GSCs) that exhibit aggressive invasion behavior in the brain. Current preclinical GBM invasion assays using mouse brain xenografts are time consuming and less efficient. Here, we demonstrate an array of methods that allow rapid

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