Anti-Cytomegalovirus Antibody, clone 8B1.2 (ASR)

Affinity purified immediate early antigen from MRC-5 cells infected with CMV AD169 (ATCC).

ELISA 1:800

Western Blot: 1:200. Recognizes approximately a 70kDa band.

Immunofluorescence: 1:200-1:400.

Immunohistochemistry 1:200-1:800. See general protocols online for guidelines.

Works on paraffin embedded tissue sections.

FACS: See online protocol.

Final working dilutions and protocols must be determined by end user.

Reacts with an immediate early non-structural antigen of 68-72 kDa. This antigen can be detected 1 hour after infection exhibiting an intranuclear staining pattern. This staining reaches a peak at 10-12 hours. This antigen persists and can be detected throughout the complete CMV infection cycle.

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Cytomegalovirus Control Slides, Catalogue Number 5027-5

This product is intended for use by IVD manufacturers.
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