产品名称
Anti-Cytomegalovirus Antibody, late Antigen, clone 1G5.2, clone 1G5.2, Chemicon®, from mouse
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1G5.2, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
isotype
IgG2a
suitability
not suitable for Western blot
shipped in
wet ice
Quality Level
Application
Detect Cytomegalovirus using this Anti-Cytomegalovirus Antibody, late antigen, clone 1G5.2 validated for use in FC, IF, IH(P).
Immunochemistry.
IFA at 1:400-1:800 on acetone fixed cells.
Works on paraffin embedded tissue sections.
Dilute with buffer pH 7.4-7.6 to desired working volume.
For extensive dilution, protein containing or other stabilizing medium should be used.
Final working dilutions must be determined by end user.
IFA at 1:400-1:800 on acetone fixed cells.
Works on paraffin embedded tissue sections.
Dilute with buffer pH 7.4-7.6 to desired working volume.
For extensive dilution, protein containing or other stabilizing medium should be used.
Final working dilutions must be determined by end user.
Research Category
Infectious Diseases
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral
Infectious Diseases - Viral
Biochem/physiol Actions
Reacts with a late protein. Can detect CMV infection 24 hours post-infection exhibiting a cytoplasmic staining which reaches peak intensity at >72 hours.
With CMV the antigens expressed at different times are listed as:
Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.
Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.
Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
With CMV the antigens expressed at different times are listed as:
Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.
Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.
Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Epitope: late antigen
Sucrose gradient purified CMV AD169 (ATCC).
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Preparation Note
Maintain at +4°C for up to 12 months
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Rapid ganciclovir susceptibility assay using flow cytometry for human cytomegalovirus clinical isolates.
J J McSharry, N S Lurain, G L Drusano, A L Landay, M Notka,
Antimicrobial agents and chemotherapy null
No evidence for human cytomegalovirus infection in pediatric medulloblastomas.
Jeroen F Vermeulen et al.
Neuro-oncology, 18(10), 1461-1462 (2016-08-16)
Charles S Cobbs et al.
Methods in molecular biology (Clifton, N.J.), 1119, 165-196 (2014-03-19)
An increased awareness of the potential oncomodulatory properties of human cytomegalovirus (HCMV) has evolved over the last decade. We first reported the presence of HCMV in human glioblastomas, and subsequently these findings have been corroborated by other groups. However, some
Joel Touma et al.
Microorganisms, 9(5) (2021-06-03)
Emerging evidence supports a significant association between human cytomegalovirus (HCMV) and human malignancies, suggesting HCMV as a human oncomodulatory virus. HCMV gene products are found in >90% of breast cancer tumors and seem to be correlated with more aggressive disease.
Overexpression of endothelin B receptor in glioblastoma: a prognostic marker and therapeutic target?
Suhas Vasaikar et al.
BMC cancer, 18(1), 154-154 (2018-02-08)
Glioblastoma (GBM) is the most common malignant brain tumor with median survival of 12-15 months. Owing to uncertainty in clinical outcome, additional prognostic marker(s) apart from existing markers are needed. Since overexpression of endothelin B receptor (ETBR) has been demonstrated in
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