Anti-Cytomegalovirus Antibody, early, clone 5A8.2

Affinity purified antigen from MRC-5 cells infected with CMV AD169 (ATCC).

Epitope: early

Anti-Cytomegalovirus Antibody, early, clone 5A8.2 detects level of Cytomegalovirus & has been published & validated for use in IF, WB, IH & IP.

Immunochemistry.

IFA at 1:3,000-1:6,000 on acetone fixed cells.

Does not work with paraffin embedded tissue sections.

Dilute with buffer pH 7.4-7.6 to desired working volume.

For extensive dilution, protein containing or other stabilizing medium should be used.

Final working dilutions must be determined by end user.

Reacts with Immediate Early 2 protein (IE2) and its fragments. Recognizes a can epitope near the carboxy-end that includes an epitope found in IE2 86, IE2 60 and IE2 40. In western blot recognizes proteins at apparent molecular weights of 86, 68-72, 55 and 38 kD. Can detect CMV infection 1 hour post-infection exhibiting nuclear and/or intranuclear inclusion staining with reaches peak intensity at 3 hours.


With CMV the antigens expressed at different times are listed as:

Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.

Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.

Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD

Format: Purified

Purified ascites. Supplied in PBS buffer, pH 7.4. Contains 0.01% sodium azide and carrier protein. Has been sterile filtered.

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