MAB8140
Anti-Cytomegalovirus Antibody, early, clone 5A8.2
clone 5A8.2, Chemicon®, from mouse
别名:
CMV
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
5A8.2, monoclonal
Species reactivity:
human
Application:
IF, IHC, IP, WB
Citations:
16
biological source
mouse
antibody form
ascites fluid
clone
5A8.2, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1
shipped in
wet ice
Quality Level
Immunogen
Affinity purified antigen from MRC-5 cells infected with CMV AD169 (ATCC).
Epitope: early
Application
Anti-Cytomegalovirus Antibody, early, clone 5A8.2 detects level of Cytomegalovirus & has been published & validated for use in IF, WB, IH & IP.
Immunochemistry.
IFA at 1:3,000-1:6,000 on acetone fixed cells.
Does not work with paraffin embedded tissue sections.
Dilute with buffer pH 7.4-7.6 to desired working volume.
For extensive dilution, protein containing or other stabilizing medium should be used.
Final working dilutions must be determined by end user.
IFA at 1:3,000-1:6,000 on acetone fixed cells.
Does not work with paraffin embedded tissue sections.
Dilute with buffer pH 7.4-7.6 to desired working volume.
For extensive dilution, protein containing or other stabilizing medium should be used.
Final working dilutions must be determined by end user.
Biochem/physiol Actions
Reacts with Immediate Early 2 protein (IE2) and its fragments. Recognizes a can epitope near the carboxy-end that includes an epitope found in IE2 86, IE2 60 and IE2 40. In western blot recognizes proteins at apparent molecular weights of 86, 68-72, 55 and 38 kD. Can detect CMV infection 1 hour post-infection exhibiting nuclear and/or intranuclear inclusion staining with reaches peak intensity at 3 hours.
With CMV the antigens expressed at different times are listed as:
Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.
Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.
Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
With CMV the antigens expressed at different times are listed as:
Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.
Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.
Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
Physical form
Format: Purified
Purified ascites. Supplied in PBS buffer, pH 7.4. Contains 0.01% sodium azide and carrier protein. Has been sterile filtered.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Yi-Chieh Perng et al.
Journal of virology, 85(10), 4841-4852 (2011-03-04)
In this study, we adopted a conditional protein genetic approach to characterize the role of the human cytomegalovirus (HCMV) gene UL79 during virus infection. We constructed ADddUL79, a recombinant HCMV in which the annotated UL79 open reading frame (ORF) was
Christopher B Ball et al.
mBio, 13(3), e0033722-e0033722 (2022-05-18)
Human cytomegalovirus (HCMV) immediate-early 2 (IE2) protein is a multifunctional transcription factor that is essential for lytic HCMV infection. IE2 functions as an activator of viral early genes, negatively regulates its own promoter, and is required for viral replication. The
The IE2 60-kilodalton and 40-kilodalton proteins are dispensable for human cytomegalovirus replication but are required for efficient delayed early and late gene expression and production of infectious virus.
White, Elizabeth A, et al.
Journal of virology, 81, 2573-2583 (2007)
Anthony R Fehr et al.
PLoS pathogens, 8(7), e1002789-e1002789 (2012-07-14)
The anaphase-promoting complex (APC) is an E3 ubiquitin ligase which controls ubiquitination and degradation of multiple cell cycle regulatory proteins. During infection, human cytomegalovirus (HCMV), a widespread pathogen, not only phosphorylates the APC coactivator Cdh1 via the multifunctional viral kinase
Salome Manska et al.
Microbiology spectrum, 9(2), e0053921-e0053921 (2021-09-23)
Human cytomegalovirus (HCMV) immediate-early 2 (IE2) protein is the major transactivator for viral gene expression and is required for lytic replication. In addition to transcriptional activation, IE2 is known to mediate transcriptional repression of promoters, including the major immediate-early (MIE)
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